The recommended storage temperature for this product, in powder form, is -20 °C. The stability of the material when stored at room temperature or -70 °C has not been investigated. Stock solutions should be prepared fresh, as solution stability has not been established. However, some sources indicate that stock solutions prepard in PBS may be stable when stored at -20 °C. Please see the link below to review additional informaion available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/296/016/h3506-pis-ms.pdf
H3506
Hyaluronidase from bovine testes
Type I-S, lyophilized powder, 400-1000 units/mg solid
Synonyma:
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
100 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 1 970,00 Kč |
500 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 6 060,00 Kč |
1 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 10 600,00 Kč |
5 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 20 700,00 Kč |
O této položce
Číslo CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
Specific activity:
400-1000 units/mg solid
1 970,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Type I-S
Quality Segment
form
lyophilized powder
specific activity
400-1000 units/mg solid
mol wt
~55 kDa (four subunits of 14 kDa each)
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
Application
Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.
Suitable for use in preparation of single cell suspension for sequencing.
Suitable for use in preparation of single cell suspension for sequencing.
Biochem/physiol Actions
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
Other Notes
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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hcodes
signalword
Danger
pcodes
Hazard Classifications
Resp. Sens. 1
Skladovací třída
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the storage range for the Hyaluronidase from bovine testes? Can be stored at room temp or -70? If yes what is the outdate for both temps?
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Can this be used in animal models in vivo?
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This product is NOT tested for injection into animals. The product is NOT sterile or tested for endotoxin levels.
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Is there any information available regarding whether hyaluronidase impacts body fluid crystals?
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Hyaluronidase is not expected to impact crystals. It is specific to hyaluronan or Hyaluronic acid and may be used with breast and possibly other tissues. However, crystals are typically inorganic salts, with body fluid crystals typically being pyrophosphate. Therefore, hyaluronidase should have no effect upon pyrophosphate crystals.
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Is it acceptable to use H3506 SLCH0779 directly in synovial fluid without dilution?
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It is generally not a standard practice to simply sprinkle hyaluronidase (such as H3506 SLCH0779) directly into synovial fluid. For a more formal and generally recognized method, a 0.05% solution in phosphate buffer is recommended, with one drop added per milliliter of fluid. This allows for the clarification of synovial fluids to make cell counts possible. Therefore, directly using H3506 SLCH0779 in synovial fluid without dilution may not be the most common practice.
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What does the type refer to i.e. type I-S?
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The type designations for the hyaluronidase enzyme products offered are in-house designations and do not represent any kind of biochemical significance. The types can refer to the order of addition of the product to the catalog, source, physical form, activity, tests performed, etc.
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Can I dissolve H3506 in DMEM/F12 media or PBS before use for tissue dissociation?
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It is recommended to dissolve this product at 1 mg/mL in 0.02 M phosphate buffer, pH 7, containing 77 mM sodium chloride and 0.01% BSA. Solubilizing directly into the medium has not been tested, but may be suitable. It is important to include BSA to ensure stability.
See the link below for the recommended instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/296/016/h3506-pis-ms.pdfHelpful?
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I am looking for specific inhibitor for extrasynaptic GABA Rs. The one I found in Sugma is Gaboxadol hydrochloride for ɣ containing containing. Which is the best inhibitor do you have. Is also any extrasynaptic GABA inhibitors for ALPHA subunits GABA?
1 answer-
Searching via the Gene Lookup tool on the main webpage search bar can provide a product list of various related products for any gene product, including agonists, antagonists, antibodies, proteins, RNAi and related gene editing products. Go to the main search bar, select "Genes" from the left dropdown and enter the gene name, gene symbol or gene id to pull a list of gene candidates. Next, select the appropriate gene by clicking on it - the related products will display. For example here is the list of gene products for the GABA A receptor alpha one (GABRA1) https://www.sigmaaldrich.com/US/en/genes/gabra1
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How is a solution of Hyaluronidase from bovine testes, Product H3506, stored?
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Prepare fresh solutions directly before use since stock solutions are not stable for storage.
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Can Hyaluronidase from bovine testes, Product H3506, be used on humans?
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No. None of our packaged research products are for human use.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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