PHR1939

Hydroxyproline

Pharmaceutical Secondary Standard; Certified Reference Material

• Synonyma: trans-4-Hydroxy-L-proline, (2S,4R)-4-Hydroxypyrrolidine-2-carboxylic acid, Hyp
• Empirický vzorec (Hillův zápis): C₅H₉NO₃
• Číslo CAS: 51-35-4
• Molekulová hmotnost: 131.13
• Beilstein/REAXYS Number: 81441
• EC Number: 200-091-9
• MDL number: MFCD00064320
• UNSPSC Code: 12352209
• NACRES: NA.24

Vlastnosti

• grade: certified reference material; pharmaceutical secondary standard
• Quality Level: 300
• API family: proline
• CofA: current certificate can be downloaded
• packaging: pkg of 500 mg
• mp: 273 °C (dec.) (lit.)
• application(s): pharmaceutical
• format: neat
• Storage temp.: 2-30°C
• SMILES string: O[C@H]1CN[C@@H](C1)C(O)=O
• InChI: 1S/C5H9NO3/c7-3-1-4(5(8)9)6-2-3/h3-4,6-7H,1-2H2,(H,8,9)/t3-,4+/m1/s1
• Inchi Key: PMMYEEVYMWASQN-DMTCNVIQSA-N

Popis

General description

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Pharmaceutical secondary standards for application in quality control provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Hydroxyproline, found exclusively in collagen, is formed from the hydroxylation of proline. It is used as a biomarker to measure the collagen content of various biological samples.

Application

This pharmaceutical secondary standard can also be used as follows:

• Multi-analysis of acetylcarnitine, proline, hydroxyproline, citrulline, and arginine as possible biomarkers by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in human plasma samples
• Simultaneous determination of proline and hydroxyproline in plasma samples by (LC-MS/MS using a new fluorescent reagent for pre-column derivatization
• Development of a micellar electrokinetic chromatography (MEKC) method to measure hydroxyproline in various dairy products upon in-capillary derivatization
• Quantitative analysis of free prolyl dipeptides, proline, and hydroxyproline by flow-gated capillary electrophoresis combined with laser-induced fluorescence detector from unhydrolyzed urine samples

Other Notes

Natural constituent of animal structural proteins such as collagen and elastin.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP and BP primary standards, where they are available.

Footnote

To see an example of a Certificate of Analysis for this material enter LRAC2898 in the Documents slot below. This is an example certificate only and may not be the lot that you receive.

Recommended products

Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1