COLLP-RO

Collagenase P

from Clostridium histolyticum

• UNSPSC Code: 12352204
• Číslo EC: 3.4.24.3 (BRENDA, IUBMB)
• NACRES: NA.54
• Biological source: Clostridium histolyticum
• Concentration: 0.5-2.5 mg/mL

Vlastnosti

• biological source: Clostridium histolyticum
• sterility: non-sterile
• form: lyophilized
• collagenase activity: >1.5 U/mg (Lyophilizate, Collagenase activity)
• packaging: pkg of 1 g (11249002001), pkg of 100 mg (11213857001), pkg of 2.5 g (11213873001), pkg of 500 mg (11213865001)
• manufacturer/tradename: Roche
• concentration: 0.5-2.5 mg/mL
• technique(s): tissue processing: suitable
• color: light brown to brown
• solubility: water: soluble
• NCBI accession no.: WP_138210331
• application(s): life science and biopharma
• foreign activity: Clostripain 24.099 U/mg, Protease 4.4 U/mg (Proteases), Trypsin 53.428 U/mg (with BAEE)
• storage temp.: 2-8°C

Popis

General description

Collagenase P is an enzyme mixture prepared from the extracellular culture filtrate of a special Clostridium histolyticum strain.Collagenase P is a research biochemical, not manufactured under the US good manufacturing practices (GMP) regulations.
Note: Collagenase P is a non-sterile preparation of the culture supernatant of Clostridium histolyticum. It is neither designed nor intended for isolation of pancreatic islets for transplantation into humans and must not be used for such purposes.

Application

Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Because of its high collagenase activity Collagenase P is particularly suitable for the isolation of pancreatic islets from mouse and rat (function tested). The preparation is also suitable for the isolation of adipocytes from epididymal fat pads of rats.[1][2]

Biochem/physiol Actions

Enzyme activity:
Collagenase activity: >1.5U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase P has a very high collagenase activity (>1.5U/mg), and is function tested for the isolation of mouse pancreatic islets.
Specificity: Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.

Features and Benefits

Contents:
Lyophilizate, nonsterile

Preparation Note

Activator: Ca²⁺
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Working concentration: 0.5 to 2.5mg/ml
0.5 to 1.5mg/ml for the isolation of pancreatic islets from mouse and rat pancreas.
Approximately 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C. Prepare appropriate aliquots and avoid repeated freezing and thawing.

Reconstitution in any balanced salt solution (e.g., HBSS)

Store dry

Other Notes

For life science research only. Not for use in diagnostic procedures.

Bezpečnostní informace

• pictograms: GHS07,GHS08
• signalword: Danger
• pcodes: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash
• hcodes: H315,H319,H334,H335