484235

p-Nitroblue Tetrazolium Chloride

≥98% (TLC), crystalline solid, nitric oxide synthase inhibitor, Calbiochem^®

• Synonyma: p-Nitroblue Tetrazolium Chloride, NBT, Nitro BT
• Empirický vzorec (Hillův zápis): C₄₀H₃₀Cl₂N₁₀O₆
• Číslo CAS: 298-83-9
• Molekulová hmotnost: 817.64
• MDL number: MFCD00012159
• UNSPSC Code: 12171500
• NACRES: NA.77

Vlastnosti

• Název produktu: p-Nitroblue Tetrazolium Chloride, NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC₅₀ = 3-4 µM).
• Quality Level: 100
• description: RTECS - XF8045000
• assay: ≥98% (TLC)
• form: crystalline solid
• potency: 3-4 μM IC₅₀
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: yellow
• solubility: methanol: soluble; water: soluble
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [Cl-].[Cl-].[n+]2([n](nc(n2)c8ccccc8)c3c(cc(cc3)c4cc(c(cc4)[n]5[n+](nc(n5)c7ccccc7)c6ccc(cc6)[N+](=O)[O-])OC)OC)c1ccc(cc1)[N+](=O)[O-]
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• InChI key: FSVCQIDHPKZJSO-UHFFFAOYSA-L

Popis

General description

NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC₅₀ = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).

Biochem/physiol Actions

Primary Target
NOS

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.

Other Notes

Pistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis.16, 29.
Libon, C., et al. 1993. J. Leukoc. Biol.53, 93.
Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
Nichols, K., et al. 1992. Neuroscience51, 791.
Nichols, K., et al. 1992. Neuroscience51, 791.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• pictograms: GHS07
• signalword: Warning
• hcodes: H302,H315,H319,H335
• pcodes: P261 - P264 - P271 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable