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RDD029

Sigma-Aldrich

Tiocianato de guanidina

for molecular biology, free-flowing, Redi-Dri™, ≥99.0%

Sinónimos:

Rodanuro de guanidinio, Tiocianato de guanidinio

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About This Item

Fórmula lineal:
NH2C(=NH)NH2 · HSCN
Número de CAS:
593-84-0
Peso molecular:
118.16
Beilstein/REAXYS Number:
3563461
MDL number:
MFCD00013027
UNSPSC Code:
12352107
NACRES:
NA.28

product line

Redi-Dri™

Quality Level

100

assay

≥99.0%

quality

for molecular biology
free-flowing

mp

120-122 °C (lit.)

foreign activity

DNAse, none detected
RNAse, none detected

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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General description

Guanadine thiocyanate (GTC) is a chaotropic agent and strong denaturant that is used in research for protein degredation in vitro. This is useful for DNA or RNA isolation and purification. GTC is a key reagent for inactivation of endogenous DNases and RNase for molecular biology techniques used to isolate nucliec acids from tissues, cells, or viral genomes.

Application

Guanidine thiocyanate is commonly used for research:

  • to denature proteins in cell lysis buffer
  • to isolate and purify RNA from cells and tissues
  • to extract DNA from cellular and viral genomes
Agente caotrópico y desnaturalizante fuerte; solubiliza las células.

Biochem/physiol Actions

Guanadine thiocyanate is strong protein denaturant commonly combinded with phenol to lyse tissues, cells, and proteins during RNA isolation assay.

Features and Benefits

  • Redi-Dri™ makes working with hygroscopic chemicals easier
  • Controls moisture and powder remains free-flowing
  • Safer to handle and weigh out for routine use in lab
  • Eliminates clumps and maintains product moisture and quality attributes

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

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Referencia del producto
Descripción
Precios

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

EUH032

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
WXBD5221V
WXBD2742V
WXBD3018V
WXBD3100V
WXBD1410V

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Martin Roszkowski et al.
Frontiers in cell and developmental biology, 9, 648274-648274 (2021-05-11)
The extraction of high-quality ribonucleic acid (RNA) from tissues and cells is a key step in many biological assays. Guanidinium thiocyanate-phenol-chloroform (AGPC) is a widely used and efficient method to obtain pure RNA from most tissues and cells. However, it
Peter L Roberts et al.
Biologicals : journal of the International Association of Biological Standardization, 35(4), 343-347 (2007-05-23)
Virus inactivation by a number of protein denaturants commonly used in gel affinity chromatography for protein elution and gel recycling has been investigated. The enveloped viruses Sindbis, herpes simplex-1 and vaccinia, and the non-enveloped virus polio-1 were effectively inactivated by
Serazhutdin A Abdullaev et al.
Radiation and environmental biophysics, 54(3), 297-304 (2015-05-04)
Investigation of cell-free DNA (cf-DNA) in body fluids, as a potential biomarker for assessing the effect of ionizing radiation on the organism, is of considerable interest. We investigated changes in the contents of cell-free mitochondrial DNA (cf-mtDNA) and cell-free nuclear

Artículos

Redi-Dri™ – The World of Free-flowing Hygroscopic Products

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

Redi-Dri™ – The World of Free-flowing Hygroscopic Products

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

Redi-Dri™ – The World of Free-flowing Hygroscopic Products

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

Redi-Dri™ – The World of Free-flowing Hygroscopic Products

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

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