G6639

Tiocianato de guanidina

≥97% (titration)

• Sinónimos: Rodanuro de guanidinio, Tiocianato de guanidinio
• Fórmula lineal: NH₂C(=NH)NH₂ · HSCN
• Número de CAS: 593-84-0
• Peso molecular: 118.16
• Beilstein/REAXYS Number: 3563461
• EC Number: 209-812-1
• MDL number: MFCD00013027
• UNSPSC Code: 12352209
• PubChem Substance ID: 24895264
• NACRES: NA.55

Propiedades

• biological source: synthetic
• Quality Level: 200
• assay: ≥97% (titration)
• form: powder or crystals
• technique(s): RNA extraction: suitable
• color: white to off-white
• mp: 120-122 °C (lit.)
• solubility: water: 4 M, clear, colorless
• anion traces: chloride (Cl^-): ≤2.5%
• SMILES string: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• InChI key: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Descripción

Application

Guanidine thiocyanate has been used:

• as a denaturing agent to study its effects on surface activity of globular protein
• for the extraction of total RNA from primary hepatocytes
• as a component of the incubating buffer for extraction of DNA from human bone
• as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA
• as a component of lysis solution for extraction of RNA from rat pancreatic tissues

Agente caotrópico y desnaturalizante fuerte; solubiliza las células.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.

Información de seguridad

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302 + H312 + H332,H314,H412
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• supp_hazards: EUH032
• Storage Class: 8B - Non-combustible corrosive hazardous materials
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves