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Key Documents

B9139

Sigma-Aldrich

Biotin-dextran

mol wt 10,000, Lysine-fixable

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About This Item

MDL number:
UNSPSC Code:
12352201
eCl@ss:
34058011
NACRES:
NA.25

conjugate

biotin conjugate

form

powder

mol wt

10,000

extent of labeling

~2 mol per mol

color

white

solubility

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

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Application

Biotin-dextran is used in tissue staining to prepare neurons for detection by avidin-conjugated detection systems. It is taken up by neuronal processes and transported bi-directionally. It infiltrates axon boutons in the anterograde direction and dendritic processes in the retrograde direction. Staining can be observed in fixed and sectioned tissue from two days to two weeks after biotin-dextran is injected into the brain or spinal cord. It can also be applied to cut nerve tracts. Biotinylated neurons are detected by either light or electron microscopy following incubation with avidin-horseradish peroxidase conjugate and the electron-dense substrate 3,3′-diaminobenzidine (DAB).

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Victoria L Cressman et al.
The Journal of comparative neurology, 518(14), 2693-2709 (2010-05-28)
Transformations in affective and social behaviors, many of which involve amygdalar circuits, are hallmarks of adolescence in many mammalian species. In this study, using the rat as a model, we provide the first evidence that afferents of the basal amygdala
Shih-Yen Tsai et al.
Stroke, 42(1), 186-190 (2010-11-23)
we have shown that anti-Nogo-A immunotherapy to neutralize the neurite growth inhibitory protein Nogo-A results in functional improvement and enhanced plasticity after ischemic stroke in the adult rat. The present study investigated whether functional improvement and neuronal plasticity can be
Jia Liu et al.
Journal of the neurological sciences, 325(1-2), 127-136 (2013-01-16)
The stem cell-based experimental therapies are partially successful for the recovery of spinal cord injury (SCI). Recently, acellular spinal cord (ASC) scaffolds which mimic native extracellular matrix (ECM) have been successfully prepared. This study aimed at investigating whether the spinal
Yang-Can Duan et al.
Pflugers Archiv : European journal of physiology, 459(1), 1-9 (2009-07-25)
This study is to determine whether the nucleus of solitary tract (NTS) is an important component of the central neurocircuitry of the cardiac sympathetic afferent reflex (CSAR) and whether the gamma-aminobutyric acid (GABA) in the NTS modulates the CSAR. Renal
Karen M Fisher et al.
The Journal of comparative neurology, 526(15), 2373-2387 (2018-07-18)
The corticospinal tract (CST) forms the major descending pathway mediating voluntary hand movements in primates, and originates from ∼nine cortical subdivisions in the macaque. While the terminals of spared motor CST axons are known to sprout locally within the cord

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