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46944

Isotiocianato de fluoresceína-dextrano

average mol wt 4,000, (FITC:Glucose = 1:250)

Sinónimos:

FITC- Dextrano 4, FITC–Dextrano

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Talla/SKUDisponibilidadPrecio
100 mg

Disponible para enviar HOYdesdePUDAHUEL

$251.000
500 mg

Disponible para enviar HOYdesdePUDAHUEL

$594.000

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Número CAS:
60842-46-8
UNSPSC Code:
12352201
NACRES:
NA.32
MDL number:
MFCD00131092

$251.000

Disponible para enviar HOYDetalles

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Quality Segment

100

form

powder

mol wt

average mol wt 4,000

composition

(FITC:Glucose = 1:250)

concentration

90-100 % (w/w) (TRITC DEXTRAN)

extent of labeling

~0.004 mol/mol FITC glucose

color

orange to dark orange, yellow to dark yellow

solubility

H2O: 50 mg/mL, slightly hazy, orange

storage temp.

2-8°C

Application

Dextrano marcado con isotiocianato de fluoresceína para su posible uso en estudios de perfusión en animales.
El FITC-dextrano se utiliza ampliamente en la investigación de la microcirculación y la permeabilidad celular utilizando microfluorimetría. El FITC-dextrano se ha utilizado para estudiar la porosidad de las paredes celulares vegetales y la permeabilidad por capilaridad. Se ha demostrado que las proteínas plasmáticas no se unen al FITC-dextrano.
El FITC-dextrano se utiliza en investigación cardiovascular, microcirculatoria, de la perfusión, de la permeabilidad de monocapas celulares y de la membrana celular como compuesto fluorescente trazador del flujo que sustenta la medición de procesos como el flujo sanguíneo, el daño de las membranas, el drenaje vascular y la eliminación renal
El isotiocianato de fluoresceína-dextrano (FITC-dextrano 4) se utiliza como una sonda fluorescente para estudiar la permeabilidad celular. El FITC-dextrano 4 se utiliza para estudiar la permeabilidad de las células epiteliales, como las células intestinales y renales in vivo.

Packaging

Frasco de vidrio sin fondo. El contenido está en el interior del cono fundido insertado.

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Este artículo
FD10S52471FD2000S
Sigma-Aldrich

Sigma-Aldrich

46944

Isotiocianato de fluoresceína-dextrano

Isotiocianato de fluoresceína-dextrano average mol wt 10,000

Sigma-Aldrich

FD10S

Isotiocianato de fluoresceína-dextrano

Sigma-Aldrich

Sigma-Aldrich

52471

Isotiocianato de fluoresceína-dextrano

Isotiocianato de fluoresceína-dextrano average mol wt 2,000,000

Sigma-Aldrich

FD2000S

Isotiocianato de fluoresceína-dextrano

description

average mol wt 4,000, (FITC:Glucose = 1:250)

description

average mol wt 10,000

description

average mol wt 2,000,000

description

average mol wt 2,000,000

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

solubility

H2O: 50 mg/mL, slightly hazy, orange

solubility

water: 25 mg/mL, cloudy to hazy, yellow to orange

solubility

-

solubility

H2O: soluble 25 mg/mL

form

powder

form

powder

form

powder or crystals

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

color

yellow to dark yellow, orange to dark orange

color

yellow to yellow-orange

color

-

color

yellow to orange

extent of labeling

~0.004 mol/mol FITC glucose

extent of labeling

0.003-0.020 mol FITC per mol glucose

extent of labeling

0.001-0.020 mol FITC per mol glucose (substitution)

extent of labeling

0.003-0.020 mol FITC per mol glucose

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pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Lot/Batch Number

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Questions

1–7 of 7 Questions  

  1. Can FITC-dextran 4KDa be view through florescence microscope to assess the gut permeability of Balb/c mice?

    1 answer

    1. Unfortunately, there is no internal data related to using this product to assess intestinal permeability. However, FITC-Dextran 4 was previously cited in this context. Kindly review the following citations:

      Tagesson C, Sjödahl R, Thorén B. Passage of molecules through the wall of the gastrointestinal tract. I. A simple experimental model. Scand J Gastroenterol. 1978;13(5):519-24. doi: 10.3109/00365527809181758. PMID: 705246.

      Ahmad R, Rah B, Bastola D, Dhawan P, Singh AB. Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching. Sci Rep. 2017 Jul 11;7(1):5125. doi: 10.1038/s41598-017-04989-8. PMID: 28698546; PMCID: PMC5505957.

      Voetmann LM, Rolin B, Kirk RK, Pyke C, Hansen AK. The intestinal permeability marker FITC-dextran 4kDa should be dosed according to lean body mass in obese mice. Nutr Diabetes. 2023 Jan 5;13(1):1. doi: 10.1038/s41387-022-00230-2. PMID: 36604407; PMCID: PMC9816099.

      Helpful?

  2. We would like to use this Product 46944-500MG for in vivo barrier integrity assays. Is there any data on the in vivo stability in lamina propria immune cells? We do not want the FITC Dextran Assay to interfere with Flow Cytometry Analysis.

    1 answer

    1. This product is not tested for the specific application, and data on in vivo stability in lamina propria immune cells is not available. A literature search is recommended for information regarding this application.

      Helpful?

  3. Are there any fluorescent hydrophobic substitutes of similar size to FITC dextran (4,000 amu) available, considering regular dextran lacks fluorescence and is hydrophilic?

    1 answer

    1. There is only one dextran conjugated with a hydrophobic fluorescent dye. The TRITC dextran, T4037, has an average dextran weight of 4,400 amu and TRITC is slightly more hydrophobic than FITC.

      Helpful?

  4. What is the recommended storage method for stock solutions of item 46944, Fluorescein isothiocyanate-dextran?

    1 answer

    1. Once Fluorescein isothiocyanate-dextran (item 46944) is in solution, it is recommended to store it at -20ºC and utilize it within 3 months to maintain its potency. To prevent multiple freeze/thaw cycles, it is advisable to aliquot the solution.

      Helpful?

  5. At what excitation and emission wavelengths should Product No. 46944, Fluorescein isothiocyanate-dextran, be read?

    1 answer

    1. The excitation maximum for FITC-dextran is 490 nm, while the emission maximum is 520 nm.

      Helpful?

  6. After reconstitution, how can we sterilise the dextran solution?

    1 answer

    1. The FITC-Dextran solution can be sterilized by filtration.

      Helpful?

  7. What is the stability of FITC-dextran in vivo?

    1 answer

    1. These products are not tested on animals. Reports suggest a 24-hour in vivo stability.

      Helpful?

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