203118

Biotin-NHS, Water-Soluble

• Sinónimos: Biotin-NHS, Water-Soluble, Sulfobiotin-NHS, Sulfosuccinimidobiotin
• Fórmula empírica (notación de Hill): C₁₄H₁₈N₃O₈S₂ · Na
• Peso molecular: 443.43
• UNSPSC Code: 41116133
• NACRES: NA.41

Propiedades

• form: solid
• Quality Level: 100
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• color: off-white
• solubility: DMSO: 10 mg/mL; distilled water: 4 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

General description

Reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.

Water-soluble form of biotin-NHS. Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Analysis Note

homogeneous single spot by TLC

Other Notes

Lisanti, M.P., et al. 1989. J. Cell Biol.109, 2145.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable