In situ formation of H2O2 for P450 peroxygenases.

In situ formation of H2O2 for P450 peroxygenases.

Bioorganic & medicinal chemistry (2014-07-06)

Caroline E Paul, Ekaterina Churakova, Elmer Maurits, Marco Girhard, Vlada B Urlacher, Frank Hollmann

ABSTRACT

An in situ H2O2 generation approach to promote P450 peroxygenases catalysis was developed through the use of the nicotinamide cofactor analogue 1-benzyl-1,4-dihydronicotinamide (BNAH) and flavin mononucleotide (FMN). Final productivity could be enhanced due to higher enzyme stability at low H2O2 concentrations. The H2O2 generation represented the rate-limiting step, however it could be easily controlled by varying both FMN and BNAH concentrations. Further characterization can result in an optimized ratio of FMN/BNAH/O2/biocatalyst enabling high reaction rates while minimizing H2O2-related inactivation of the enzyme.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

D2650

Sigma-Aldrich

Dimetil solfossido, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

472301

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Dimetil solfossido, ACS reagent, ≥99.9%

D8418

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Dimetil solfossido, for molecular biology

319902

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Etilacetato, ACS reagent, ≥99.5%

34869

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Dimetil solfossido, suitable for HPLC, ≥99.7%

D2438

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Dimetil solfossido, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

T1503

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Trizma^® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline

34858

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Etilacetato, suitable for HPLC, ≥99.7%

673811

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Dietiletere, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor

D5879

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Dimetil solfossido, ReagentPlus^®, ≥99.5%

309966

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Dietiletere, suitable for HPLC, ≥99.9%, inhibitor-free

650528

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Etilacetato, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%

T6066

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Trizma^® base, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

346136

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Dietiletere, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor

D4540

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Dimetil solfossido, ≥99.5% (GC), suitable for plant cell culture

41640

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Dimetil solfossido, puriss. p.a., ACS reagent, ≥99.9% (GC)

92361

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Chlorotrimethylsilane, ≥98.0% (GC)

T1378

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Sigma 7-9^®, ≥99% (titration), crystalline

T6687

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Tromethamine, meets USP testing specifications

439169

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Etilacetato, suitable for HPLC, ≥99.8%

93362

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Trizma^® base, BioUltra, for molecular biology, ≥99.8% (T)

32203

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Dietiletere, contains BHT as inhibitor, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)

33211

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Etilacetato, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

41639

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Dimetil solfossido, BioUltra, for molecular biology, ≥99.5% (GC)

386529

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Chlorotrimethylsilane, purified by redistillation, ≥99%

93352

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Trizma^® base, ≥99.0% (T)

27227

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Etilacetato, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99.5% (GC)

W261408

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T6791

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276855

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