Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

Visualizza tutta la documentazione

X4875

Sigma-Aldrich

Xanthine Oxidase from bovine milk

Grade IV, ammonium sulfate suspension, ≥0.1 units/mg protein

Sinonimo/i:

XOD, Xanthine:oxygen oxidoreductase

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali

Scegli un formato

10 UNITS
CHF 86.00
40 UNITS
CHF 218.00
120 UNITS
CHF 527.00

CHF 86.00

Check Cart for Availability
Richiedi un ordine bulk
Tutte le immagini(1)

Scegli un formato

Cambia visualizzazione
10 UNITS
CHF 86.00
40 UNITS
CHF 218.00
120 UNITS
CHF 527.00

About This Item

Numero CAS:
9002-17-9
Classificazione EC (Enzyme Commission):
1.17.3.2.
Numero CE:
232-657-6
Numero MDL:
MFCD00082145
Codice UNSPSC:
12352204
NACRES:
NA.54

CHF 86.00

Check Cart for Availability
Richiedi un ordine bulk

Origine biologica

bovine milk

Tipo

Grade IV

Stato

ammonium sulfate suspension

Attività specifica

≥0.1 units/mg protein

Temperatura di conservazione

2-8°C

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

Formerly E.C. 1.1.3.22
Research area: Cell CycleXanthineoxidase (XO) is a homodimer. It is made up of two sub-units each made up of onemolybdenum and two iron-sulphur (2Fe-2S) centers.[1]

Applicazioni

Xanthine Oxidase from bovine milk has been used in superoxide dismutaseactivity assay.[2][3]Xanthine oxidase may be suitable for producing amperometric biosensors and designing-flavonoid-type inhibitors against XO for treating inflammatory diseases, gout, and hyperuricemia. It may also be used in the synthesis of superoxide because of aldehyde oxidation.

Azioni biochim/fisiol

Although xanthine oxidase is found widely distributed in multiple tissues, it is highly expressed in endothelial and epithelial cells. Its expression was also increased in infected tissues where it was shown to have an anti-microbial effect due to its ability to generate reactive oxygen species. [4]Xanthineoxidase plays an important role in catalyzing the hydroxylation ofhypoxanthine to Xanthine. Later, xanthine is converted to uric acid isexcreted by the kidneys.[1]
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Definizione di unità

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Stato fisico

Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate

Risultati analitici

Protein determined by biuret

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
0000299810
0000299810
0000292863
0000292865
0000292864

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Cerca un COA

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

M B Betancor et al.
Fish physiology and biochemistry, 41(3), 705-720 (2015-03-10)
In vertebrates, selenium (Se) is an essential micronutrient for vertebrates that is involved in antioxidant protection and thyroid hormone regulation among other roles and functions through its incorporation into proteins, the selenoproteins. Long-chain polyunsaturated fatty acids, such as docosahexaenoic acid
Xanthine oxidase: isolation, assays of activity, and inhibition
Kostic D, et al.
Journal of Chemistry, 2015(1), 294858-294858 (2015)
João L Serrano et al.
Biomedicines, 9(10) (2021-10-24)
Xanthine oxidase (XO) is the enzyme responsible for the conversion of endogenous purines into uric acid. Therefore, this enzyme has been associated with pathological conditions caused by hyperuricemia, such as the disease commonly known as gout. Barbiturates and their congeners
Evolution of the anticholinesterase, antioxidant, and anti-inflammatory activity of Epilobium angustifolium L. infusion during in vitro digestion
Szwajgier D, et al.
Journal of functional foods, 85, 104645-104645 (2021)
Szymon Byczkiewicz et al.
Nutrients, 16(11) (2024-06-19)
In the present study, the effect of the addition of quince and collagen type I and III to dessert chocolate on its functional properties was determined. The study evaluated the antioxidant potential of the tested formulations using the FRAP method
Visualizza tutti i documenti correlati

Articoli

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Questions

  1. Hello, Is there a way to find out how much 2.3M (NH4)2SO4 was used to suspend 10 Units of Xanthine Oxidase? Specifically, what is the ratio of units Xanthine Oxidase (or mg) to volume needed to create this suspension using lyophilized powder? Thank you!

    1 answer

    1. The volume of a given package size varies from lot to lot. The minimum activity of this product is 0.1 units per milligram of protein. The minimum protein concentration is 5.0 mg/mL. As an example, a lot reporting an activity of 0.3 units per milligram protein at a concentration of 13.8 mg protein per milliliter, will have a volume of 2.3 mL for the 10 Unit package size. This is determined by using the following calculation:
      10U / (0.3U/mg) = 30mg / (13.8mg/ml) = 2.3 ml volume

      The lot-specific volume may be determined by data provided in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/x4875#product-documentation

      Helpful?

Reviews

No rating value

Active Filters

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.