For optimal solubility, this enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution. Some customers have reported issues with solubility when using other buffers, such as Tris and water. Although an enzymatic assay utilized water, the concentration in that case was very low (0.125 units/ml).
N2415
Nucleoside Phosphorylase bacterial
recombinant, expressed in E. coli, ≥10 units/mg protein
Sinonimo/i:
PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase
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Informazioni su questo articolo
Numero CAS:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-857-3
MDL number:
Specific activity:
≥10 units/mg protein
Assay:
≥80% protein basis (biuret)
Recombinant:
expressed in E. coli
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Servizio Tecnico
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Permettici di aiutartirecombinant
expressed in E. coli
Quality Segment
assay
≥80% protein basis (biuret)
form
lyophilized powder
specific activity
≥10 units/mg protein
sequence note
MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE
UniProt accession no.
storage temp.
−20°C
SMILES string
[n]2(c3ncncc3nc2)[C@@H]1O[C@@H]([C@H]([C@H]1O)O)CO
InChI
1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1
InChI key
MRWXACSTFXYYMV-FDDDBJFASA-N
Gene Information
Escherichia coli ... deoD(945654)
Application
Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.[1]
Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.
Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.
Biochem/physiol Actions
Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.
Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
Preparation Note
This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution.
Other Notes
One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.
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Questo articolo | |||
|---|---|---|---|
| Gene Information Escherichia coli ... deoD(945654) | Gene Information - | Gene Information Escherichia coli K12 ... trxB(949054) | Gene Information - |
| specific activity ≥10 units/mg protein | specific activity ≥25 units/mg protein | specific activity >25 units/mg protein (Bradford) | specific activity ≥20 units/mg protein |
| assay ≥80% protein basis (biuret) | assay - | assay - | assay - |
| form lyophilized powder | form liquid | form ammonium sulfate suspension | form lyophilized powder |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant - |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. | UniProt accession no. |
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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