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WTA2

Sigma-Aldrich

TransPlex® Complete Whole Transcriptom Amplification Kit

DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

Sinonimo/i:

transcriptome amplification kit

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CHF 1’110.00
50 REACTIONS
CHF 5’340.00

CHF 1’110.00

Spedizione prevista il30 marzo 2025

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10 REACTIONS
CHF 1’110.00
50 REACTIONS
CHF 5’340.00

About This Item

Codice UNSPSC:
41121800
NACRES:
NA.55

CHF 1’110.00

Spedizione prevista il30 marzo 2025

Livello qualitativo

200

tecniche

whole genome amplification: suitable

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

WTA2 is optimized to amplify RNA from formalin-fixed, paraffin-embedded (FFPE) and other damaged or degraded samples. Whole Transcriptome Amplification (WTA) technology, allows for representative amplification of low nanogram quantities of total RNA in less than 4 hours without 3′-bias. Amplification products are suitable for applications such as qPCR, micro array analysis, and cloning. The WTA2 kit contains the polymerase needed to amplify the cDNA library.

Applicazioni

Complete Whole Transcriptome Amplification Kit is used for the following applications:
  • To establish a protocol for the simultaneous analysis of DNA and RNA viruses present in pig feces using process controlled deep sequencing.[1]
  • Reverse transcription and cDNA amplification[2][3][4][5][6][7]
  • For the synthesis and amplification of cDNA library using Genomic RNA released from immunocaptured PPV particles[8]
  • Nucleic Acid Preparation and Deep Sequencing (The extracted nucleic acids were randomly primed for cDNA synthesis)[9]
Suitable for use with downstream applications including:
  • qPCR
  • microarray analysis
  • cloning

Caratteristiche e vantaggi

  • Achieve up to 10,000x amplification in less than 4 hours with less than 30 minutes of "hands on" time required
  • Only 20 pg of total RNA template is required to amplify suitable cDNA for microarray profiling
  • Contains all needed components for cDNA amplification
  • Achieve linear amplification of expressed genes and exons without 3′ or 5′ bias
  • Effectively amplifies single cell or low input RNA, including mRNA and total RNA from any animal, plant, or microorganism

Principio

The WTA2 process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. During this process, displaced single strands serve as new templates for primer annealing and extension. The resultant cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence. The 2nd step amplifies the cDNA library by PCR using WTA2 polymerase and a universal end primer to produce WTA2 product.

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • Library Synthesis Enzyme
  • Library Synthesis Solution
  • Amplification Mix
  • Library Synthesis Buffer
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS
  • Amplification Enzyme
  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology ReagentSDS

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

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Certificati d'analisi (COA)

Lot/Batch Number
SLCQ5847
SLCP9475
SLCQ8573
SLCQ8820
SLCP6950

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Bert Vanmechelen et al.
Scientific reports, 8(1), 11171-11171 (2018-07-26)
The family Arteriviridae harbors a rapidly expanding group of viruses known to infect a divergent group of mammals, including horses, pigs, possums, primates, and rodents. Hosts infected with arteriviruses present with a wide variety of (sub) clinical symptoms, depending on
Richard J Hall et al.
Journal of virological methods, 195, 194-204 (2013-09-17)
The discovery of new or divergent viruses using metagenomics and high-throughput sequencing has become more commonplace. The preparation of a sample is known to have an effect on the representation of virus sequences within the metagenomic dataset yet comparatively little
A Bal et al.
BMC infectious diseases, 18(1), 537-537 (2018-10-31)
In recent years, metagenomic Next-Generation Sequencing (mNGS) has increasingly been used for an accurate assumption-free virological diagnosis. However, the systematic workflow evaluation on clinical respiratory samples and implementation of quality controls (QCs) is still lacking. A total of 3 QCs
Anna Sheveleva et al.
Virus genes, 47(2), 385-388 (2013-07-03)
The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. This region was amplified using
Distinct degenerative phenotype of articular cartilage from knees with meniscus tear compared to knees with osteoarthritis
Rai MF, et al.
Osteoarthritis and Cartilage, 27, 945-955 (2019)
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Articoli

WTA2 Amplicon Modification for NGS

Transplex Whole Transcriptome Amplification (WTA2) precisely amplifies RNA maintaining transcript levels in test and reference samples.

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

Protocolli

Integration of Sigma® TransPlex® with Agilent Microarray

TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

Contenuto correlato

Whole Transcriptome Amplification FAQs

Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications

Questions

  1. What are the differences between WTA1 and WTA2?

    1 answer

    1. WTA2 is designed to amplify RNA from formalin-fixed, paraffin-embedded (FFPE) and other damaged or degraded samples. Here are the main differences between the two kits:

      1. WTA1 was developed by Rubicon. WTA2 was developed by Sigma-Aldrich.
      2. The library synthesis primers for WTA2 are different and can prime more frequently, which is crucial for degraded RNAs.
      3. The library synthesis conditions are different due to the change in the primers.
      4. There are improved cycling conditions in WTA2 to optimize amplification of both high AT templates and high GC templates.
      5. WTA1 does not contain an amplification enzyme, which needs to be supplied separately. WTA2 includes the amplification enzyme.

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