This enzyme is a homodimer comprised of 27 kDa subunits.
T4330
Endonucleasi
recombinant, expressed in E. coli
Sinonimo/i:
Endonuclease from Serratia marcescens
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50000 UNITS
CHF 341.00
CHF 341.00
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Cambia visualizzazione
50000 UNITS
CHF 341.00
About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
CHF 341.00
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Prodotti consigliati
Origine biologica
Serratia marcescens
Livello qualitativo
Ricombinante
expressed in E. coli
Stato
liquid
Concentrazione
≥200,000 units/mL
tecniche
DNA purification: suitable
Compatibilità
suitable for cell lysis
applicazioni
life science and biopharma
Temperatura di conservazione
−20°C
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Categorie correlate
Descrizione generale
Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.[1]
Applicazioni
Utilizzato per rimuovere gli acidi nucleici dai campioni di proteine.
Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification[2]. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.[3]
Turbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating. [4]
Azioni biochim/fisiol
Digerisce DNA ed RNA nativi o denaturati con calore.
Endonuclease from Serratia marcescens is effective against both single- and double-stranded DNA and RNA. It mediates the digestion of the 3′ O—P bond resulting in oligonucleotides ending with 5′ monophosphate. The activity of this enzyme is known to be less affected by the reducing and chaotropic agents. It is highly stable at room temperature. This endonuclease eliminates the undesired nucleic acids in downstream processing.[1]
Turbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA.[5]
Definizione di unità
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C.
Stato fisico
Supplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaCl
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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