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SAB3701171

Sigma-Aldrich

Anti-Mouse IgG1 (γ-chain specific)-Peroxidase antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Sinonimo/i:

HRP

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46
Prezzi e disponibilità al momento non sono disponibili

Origine biologica

rabbit

Coniugato

peroxidase conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Stato

lyophilized powder

Reattività contro le specie

mouse

tecniche

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Categorie correlate

Descrizione generale

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. IgG1 is induced due to antibody responses to membrane proteins and soluble protein antigens. It is the most abundant subclass of IgG.

Specificità

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA against other Mouse or Human heavy chain isotypes. Cross reactivity with Mouse IgG2b and IgG3 is negligible (<0.5%). Cross reactivity with Mouse IgG2a and IgM is minimal.

Immunogeno

Mouse IgG1 heavy chain

Applicazioni

Anti-Mouse IgG1 (γ-chain specific)-Peroxidase antibody produced in rabbit has been used in immunohistochemistry and Western blotting.

Proprietà fisiche

Antibody format: IgG

Stato fisico

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Ricostituzione

Reconstitute with 1.0 mL deionized water (or equivalent).

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Avvertenze

Warning

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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