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A9917

Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat

Name: Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
Brand: SIGMA
Price: 220 CHF

affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

Goat Anti-Mouse IgG (Fab specific)–HRP

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A voi/SKU	Disponibilità	Prezzo
1 mL	Per conoscere la disponibilità, visualizza il carrello	CHF 220.00

Informazioni su questo articolo

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162623

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB

Citations:

222

CHF 220.00

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Proprietà

biological source

goat

Quality Segment

300

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, western blot: 1:80,000-1:160,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Descrizione

General description

Immunoglobulin G (IgG) is a glycoprotein antibody. IgG belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.

Immunogen

Purified mouse IgG Fab fragment

Application

Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.

Anti-Mouse IgG (Fab specific)-Peroxidase antibody has been used in immunofluorescence, ELISA (enzyme-linked immunosorbent assay) and immunoblotting.
Goat Anti-Mouse IgG (Fab specific)-Peroxidase antibody can be used for western blotting assays. The product can also be used for direct ELISA (1:60,000) and IHC (1:150) applications.

Bovine CVEC and human epidermoid carcinoma A431 cell lysates were analyzed by western blot using HRP-conjugated goat anti-mouse IgG Fab specific as the secondary antibody at a 1:3000 dilution.

Biochem/physiol Actions

Immunoglobulin G (IgG) regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation, opsonization and antibody dependent cell mediated cytotoxicity in mice. IgG participates in hypersensitivity type II and type III.

Specificity of the Anti-Mouse IgG (Fab Specific)- Peroxidase is determined by immunoelectrophoresis (IEP), prior to conjugation. By IEP, single precipitation arcs are observed against normal mouse serum, mouse IgG and the Fab fragment of mouse IgG, while no reaction is observed against the Fc fragment of mouse IgG or human IgG. Binds all mouse IgGs.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Adsorbed to reduce background staining with human samples.

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Antibody adsorbed with human IgG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo	A3682	A2554	A2304
Sigma-Aldrich A9917 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat	Sigma-Aldrich A3682 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat	Sigma-Aldrich A2554 Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat	Sigma-Aldrich A2304 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
biological source goat	biological source goat	biological source goat	biological source goat
technique(s) direct ELISA: 1:60,000, western blot: 1:80,000-1:160,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150	technique(s) direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, western blot: 1:80,000-160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per lane)	technique(s) direct ELISA: 1:70,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:80,000-1:160,000 using total cell extract of Hela cells (5-10 μg/mL)	technique(s) direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue section, western blot: 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
species reactivity mouse	species reactivity mouse	species reactivity mouse	species reactivity mouse
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Classe di stoccaggio

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c