M3817
MegaCell™ RPMI-1640 Medium
without L-glutamine, liquid, sterile-filtered, suitable for cell culture
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About This Item
Codice UNSPSC:
12352207
NACRES:
NA.75
Prodotti consigliati
Sterilità
sterile-filtered
Forma fisica
liquid
tecniche
cell culture | mammalian: suitable
Impurezze
endotoxin, tested
Componenti
L-glutamine: no
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
Descrizione generale
MegaCell™ is a line of versatile media, formulated to significantly reduce the amount of serum required for cultivating mammalian cells in vitro. It is effective as a basal formulation for the growth and maintenance of adherent or suspension cell lines. When supplemented with 3% fetal bovine serum (FBS), MegaCell supports proliferative rates and maximal cell densities comparable, and in some cases superior, to the conventional basal formulation supplemented with 10% fetal bovine serum.
Applicazioni
MegaCell™ RPMI-1640 Medium has been used:
- as a component of the TSC medium for culturing trophoblast stem cells (TSC)
- as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs
- as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs
Ricostituzione
Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)
Note legali
Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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