Il terreno RPMI 1640 è stato sviluppato nel 1966 presso il Roswell Park Memorial Institute da Moore e i suoi collaboratori. Inizialmente ottenuto da una modifica del terreno 5A di McCoy per supportare la crescita di cellule linfoblastoidi in sospensione, si è visto successivamente che è in grado di supportare anche la crescita di un′ampia varietà di cellule che dipendono dall′adesione. Inizialmente ideato per essere supplementato con siero, l′RPMI 1640 si è dimostrato in grado di supportare la crescita di diverse linee cellulari anche in assenza di siero. È anche ampiamente usato in protocolli di fusione e per la crescita di cellule ibride. Questo terreno è adatto per la coltura di leucociti umani normali e neoplastici.
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.
Applicazioni
RPMI-1640 Medium has been used to incubate dissected lung tissue.[1]
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