L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled. After thawing, it will be an opalescent solution. If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization. To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed. This will expose the product to the minimal amount of warming cycles.
G2150
L-glutammina
200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma
Sinonimo/i:
L-Glutamine for cell culture
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352209
eCl@ss:
32160406
ID PubChem:
NACRES:
NA.75
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Grado
Hybri-Max™
Livello qualitativo
Sterilità
sterile-filtered
Saggio
≥99% (TLC)
Stato
solution
tecniche
cell culture | hybridoma: suitable
Impurezze
endotoxin, tested
Colore
clear colorless
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Stringa SMILE
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
ZDXPYRJPNDTMRX-VKHMYHEASA-N
Informazioni sul gene
rat ... Ggt1(116568)
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Categorie correlate
Descrizione generale
La L-glutammina è un amminoacido essenziale ed un componente fondamentale dei terreni di coltura, utilizzato come importante fonte di energia per le cellule coltivate. L-glutammina è molto stabile sia come polvere secca che come soluzione congelata. Nei terreni o in soluzione madre tuttavia, la L-glutammina si degrada in tempi relativamente rapidi. Per ottenere una prestazione ottimale, la L-glutammina viene solitamente aggiunta ai terreni prima dell′uso.
Applicazioni
The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.
Nota sulla preparazione
Prepared in cell culture grade water.
Note legali
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Applicazioni
N° Catalogo
Descrizione
Determinazione del prezzo
Prodotti correlati
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
Importance and uses of glutamine in hybridoma and mammalian cell culture
Chromatograms
application for HPLC-
Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
1 answer-
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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