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C7594

Sigma-Aldrich

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

Sinonimo/i:

blocking buffer

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About This Item

Numero CE:
232-555-1
Codice UNSPSC:
12161700
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

200

Forma fisica

powder blend

tecniche

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

Solubilità

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

Temperatura di conservazione

room temp

Categorie correlate

Descrizione generale

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Applicazioni

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Caratteristiche e vantaggi

  • Better blocking performance than dried milk or BSA
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays

Nota sulla preparazione

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

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Protocolli

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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