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C5670

Sigma-Aldrich

Cloruro di calcio

anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Sinonimo/i:

CaCl2, Calcium dichloride

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100 G
CHF 200.00
500 G
CHF 776.00

CHF 200.00

Spedizione prevista il09 aprile 2025

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Cambia visualizzazione
100 G
CHF 200.00
500 G
CHF 776.00

About This Item

Formula condensata:
CaCl2
Numero CAS:
10043-52-4
Peso molecolare:
110.98
Numero CE:
233-140-8
Numero MDL:
MFCD00010903
Codice UNSPSC:
12352207
ID PubChem:
24892577
NACRES:
NA.71
Saggio:
≥96.0%
Grado:
anhydrous
Stato:
powder

CHF 200.00

Spedizione prevista il09 aprile 2025

Richiedi un ordine bulk

Grado

anhydrous

Livello qualitativo

200

Tensione di vapore

0.01 mmHg ( 20 °C)

Nome Commerciale

BioReagent

Saggio

≥96.0%

Stato

powder

tecniche

cell culture | insect: suitable
cell culture | plant: suitable

Punto di fusione

772 °C (lit.)

Stringa SMILE

[Cl-].[Cl-].[Ca++]

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2
UXVMQQNJUSDDNG-UHFFFAOYSA-L

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Categorie correlate

Descrizione generale

Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.1,2 Extensive reviews of the experimental measurement of biological calcium have been published.3,4Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.5 The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.6 A protocol for the concentration of virus vectors that uses CaCl2 has been published.7CaCl2 has been used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli.8 The crystallization of porcine pancreatic elastase in the presence of CaCl2 in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.9 The effect of CaCl2 on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.10

Applicazioni

This product is insect cell culture tested (0.5 mg/ml) and is appropriate for insect cell culture applications. Calcium chloride is used in the preparation and transformation of competent E. coli and in the
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.

Nota sulla preparazione

This product is soluble in water (222 mg/ml, 2 M), yielding a clear, colorless solution.

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H319

Classi di pericolo

Eye Irrit. 2

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0000409821
0000409822
0000409822
0000409821
0000389969

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Maheswari Jayakannan et al.
Journal of experimental botany, 66(7), 1865-1875 (2015-01-24)
The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which
J Philippe et al.
International journal of obesity (2005), 39(2), 295-302 (2014-06-04)
A significant proportion of severe familial forms of obesity remain genetically elusive. Taking advantage of our unique cohort of multigenerational obese families, we aimed to assess the contribution of rare mutations in 29 common obesity-associated genes to familial obesity, and
Mohsen Akbari et al.
Advanced functional materials, 24(26), 4060-4067 (2014-11-21)
The fabrication of cell-laden structures with anisotropic mechanical properties while having a precise control over the distribution of different cell types within the constructs is important for many tissue engineering applications. Automated textile technologies for making fabrics allow simultaneous control
Michael A Goren et al.
Nature communications, 5, 5115-5115 (2014-10-09)
Opsin, the rhodopsin apoprotein, was recently shown to be an ATP-independent flippase (or scramblase) that equilibrates phospholipids across photoreceptor disc membranes in mammalian retina, a process required for disc homoeostasis. Here we show that scrambling is a constitutive activity of
Gemma L Carvill et al.
Neurology, 82(14), 1245-1253 (2014-03-14)
To determine the genes underlying Dravet syndrome in patients who do not have an SCN1A mutation on routine testing. We performed whole-exome sequencing in 13 SCN1A-negative patients with Dravet syndrome and targeted resequencing in 67 additional patients to identify new
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Articoli

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. What is the procedure for preparing a 2 M solution using calcium chloride and calcium chloride dihydrate?

    1 answer

    1. The molecular weight for calcium chloride dihydrate is 147.01 g/mol, so a 2 M solution would be 294 g/L. Similarly, the molecular weight for calcium chloride is 110.98 g/mole, so a 2 M solution would be 222 g/L. Therefore, a 2 M solution of calcium chloride dihydrate (such as C7902) would be 294 g/L with a molecular weight of 147.01 g/mol.

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