Unfortunately, a protocol for dilution is not available. However, the following information from the product manual may be helpful. Some types of primary human cells, especially Normal Human Epidermal Keratinocytes, respond sensitively to the calcium concentration in the culture medium. Calcium Chloride solution allows the adjustment of the optimal amount of calcium required by the cells. The Calcium Chloride (CaCl2) Solution is prepared as a solution from highly pure tissue culture tested Calcium Chloride and cell culture-grade water. It is a clear, colorless, and sterile solution. The concentration of CaCl2 is 0.5 M, and each vial contains 2 mL.
C-34006
Calcium Chloride Solution
CaCl 2-Solution (0.5 M), 2 ml
Sinonimo/i:
Calcium Chloride Liquid
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Cambia visualizzazione
2 ML
CHF 36.90
About This Item
Codice UNSPSC:
12352207
NACRES:
NA.81
Prodotti consigliati
Grado
for analytical purposes
Stato
solution
Confezionamento
pkg of 2 ml
tecniche
cell culture | mammalian: suitable
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Descrizione generale
CaCl 2-Solution
Applicazioni
Some types of primary human cells, especially Normal Human Epidermal Keratinocytes (NHEK), respond sensitively to the calcium concentration in the culture medium. Calcium Chloride solution allows the adjustment of the optimal amount of calcium required by the cells. PromoCell Calcium Chloride (CaCl2) Solution is prepared as a solution from highly pure tissue culture tested Calcium Chloride and cell culture-grade water. It is a clear colorless and sterile solution.The concentration of CaCl2 is 0.5 M.
Qualità
All lots of PromoCell Calcium Chloride Solution are subjected to comprehensive quality control tests. Each lot is routinely tested for the use with human primary cells, absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).
Stoccaggio e stabilità
Store at -20°C in the dark immediately after arrival. If stored properly, the product is stable until the expiry date stated on the label.
Mantenimento delle subcolture
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Certificati d'analisi (COA)
Lot/Batch Number
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I received this product for my master assay, and I saved it in -20 C. What should i do in order to use it and to change its concentration? Thank you so much for your time,
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