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46043

Sigma-Aldrich

Ethidium homodimer

suitable for fluorescence, ~90% (HPCE)

Sinonimo/i:

EtDi, EthD-1

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1 MG
CHF 379.00
5 MG
CHF 1’540.00

CHF 379.00


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Cambia visualizzazione
1 MG
CHF 379.00
5 MG
CHF 1’540.00

About This Item

Formula empirica (notazione di Hill):
C46H50Cl4N8
Numero CAS:
Peso molecolare:
856.75
Numero CE:
Numero MDL:
Codice UNSPSC:
12161505
ID PubChem:
NACRES:
NA.32

CHF 379.00


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Saggio

~90% (HPCE)

Stato

solid

Punto di fusione

≥250 °C (lit.)

Solubilità

DMF: soluble
DMSO: soluble
H2O: soluble

Fluorescenza

λex 493 nm in H2O
λex 528 nm; λem 617 nm in TE buffer; DNA

Compatibilità

suitable for fluorescence

Temperatura di conservazione

−20°C

Stringa SMILE

Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28

InChI

1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
ZKAJUKABUUJRLB-UHFFFAOYSA-N

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Applicazioni

Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.
Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.

Confezionamento

Bottomless glass bottle. Contents are inside inserted fused cone.

Altre note

Reagent for the fluorimetric detection of nucleic acids[1]

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - Water-react 3

Codice della classe di stoccaggio

4.3 - Hazardous materials which set free flammable gases upon contact with water

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves


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Questions

  1. If possible to send me how to prepare & use this item.

    1 answer
    1. This product has not been tested for use in any specific application. The material is soluble in DMSO, Dimethylformamide, and water. Please see the link below to review additional publications that may be helpful:
      https://www.sciencedirect.com/topics/chemistry/ethidium-homodimer

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