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P4864

Sigma-Aldrich

Propidium iodide solution

≥94% purity, solution (1.0 mg/ml in water)

Sinonimo/i:

3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide

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About This Item

Formula empirica (notazione di Hill):
C27H34I2N4
Numero CAS:
Peso molecolare:
668.39
Beilstein:
3843838
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47

product name

Propidium iodide solution,

Saggio

≥94%

Livello qualitativo

Forma fisica

liquid

Colore

faint orange to very dark orange, and Faint Red to Very Dark Red and Faint Purple to Very Dark Purple and Orange-Red and Red-Orange

Solubilità

water: 1 mg/mL

εmax

5400-6400 at 491-495 nm in phosphate buffer

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

2-8°C

Stringa SMILE

[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
XJMOSONTPMZWPB-UHFFFAOYSA-M

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Descrizione generale

Propidium iodide solution is used to assess neuronal death.

Applicazioni

Propidium iodide solution has been used:
  • in staining DNA
  • in the viability staining method to study islet viability
  • to visualize F-actins and nuclei in mesenchymal stem cell sheets

Membrane impermeant, phenanthridinium intercalator dye excited by mercury or xenon-arc lamps or argon-ion laser. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Used as a nuclear counterstain.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves


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Variation in human islet viability based on different membrane integrity stains
Barnett MJ, et al.
Cell Transplantation, 13(5), 481-488 (2004)
Stroke E-Book: Pathophysiology Diagnosis and Management, 79-79 (2011)
Direct intramyocardial injection of mesenchymal stem cell sheet fragments improves cardiac functions after infarction
Wang CC, et al.
Cardiovascular Research, 77(3), 515-524 (2007)
Yonggang Yang et al.
Scientific reports, 5, 18583-18583 (2015-12-22)
Viability is a common issue of concern in almost all microbial processes. Fluorescence-based assays are extensively used in microbial viability assessment, especially for mixed-species samples or biofilms. Propidium iodide (PI) is the most frequently used fluorescence indicator for cell viability
Paternal cyclophosphamide exposure induces the formation of functional micronuclei during the first zygotic division
Grenier L, et al.
PLoS ONE, 6(11), e27600-e27600 (2011)

Articoli

Saggi basati su cellule per valutare la proliferazione cellulare (BrdU, MTT, WST1), la vitalità cellulare e la citotossicità nel campo della ricerca oncologica, neuroscientifica e delle cellule staminali.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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