30915
Denhardt′s solution
BioReagent, for molecular biology, lyophilized powder
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About This Item
Prodotti consigliati
Grado
Molecular Biology
for molecular biology
Nome Commerciale
BioReagent
Stato
lyophilized powder
Impurezze
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
Compatibilità
suitable for nucleic acid hybridization
Temperatura di conservazione
2-8°C
Descrizione generale
Denhardt′s solution is a mixture of high-molecular weight polymers that act as blocking agents. It is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Applicazioni
Suitable to block non-specific binding sites in western, northern and Southern blot membranes
Componenti
Each vial of Denhardt′s powder contains 50 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP).
Nota sulla preparazione
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at −20°C for up to two years.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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