- A novel functional role for MMSET in RNA processing based on the link between the REIIBP isoform and its interaction with the SMN complex.
A novel functional role for MMSET in RNA processing based on the link between the REIIBP isoform and its interaction with the SMN complex.
PloS one (2014-06-14)
Fabio Mirabella, Alexander Murison, Lauren I Aronson, Christopher P Wardell, Andrew J Thompson, Sarah J Hanrahan, Jacqueline H L Fok, Charlotte Pawlyn, Martin F Kaiser, Brian A Walker, Faith E Davies, Gareth J Morgan
PMID24923560
ZUSAMMENFASSUNG
The chromosomal translocation t(4;14) deregulates MMSET (WHSC1/NSD2) expression and is a poor prognostic factor in multiple myeloma (MM). MMSET encodes two major protein isoforms. We have characterized the role of the shorter isoform (REIIBP) in myeloma cells and identified a clear and novel interaction of REIIBP with members of the SMN (survival of motor neuron) complex that directly affects the assembly of the spliceosomal ribonucleic particles. Using RNA-seq we show that REIIBP influences the RNA splicing pattern of the cell. This new discovery provides novel insights into the understanding of MM pathology, and potential new leads for therapeutic targeting.
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Natriumchlorid, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
Natriumchlorid -Lösung, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
Natriumchlorid, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
Formaldehyd -Lösung, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
Supelco
Formaldehyd -Lösung, stabilized with methanol, ~37 wt. % in H2O, certified reference material
Alanin, European Pharmacopoeia (EP) Reference Standard