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Sigma-Aldrich

Sephadex® G-100

BioReagent, for molecular biology, DNA grade, medium

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About This Item

CAS-Nummer:
9050-94-6
MDL-Nummer:
MFCD00132235
UNSPSC-Code:
23151817
NACRES:
NA.52

Qualität

DNA grade
for molecular biology

Produktlinie

BioReagent

Form

beads

Methode(n)

buffer exchange: suitable

Aktive Matrixgruppe

phase

Quellen

1 g swells to 9-11 mL

Perlendurchmesser

40-120 μm

Porengröße

exclusion limit (25 bp dsDNA; (rA)27)

pH-Wert

2-10

Anwendung(en)

life science and biopharma

Kompatibilität

Cytiva

Fremdaktivität

DNase, RNase, none detected

Lagertemp.

room temp

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Allgemeine Beschreibung

Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Anwendung

Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.

Leistungsmerkmale und Vorteile

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Rechtliche Hinweise

Sephadex is a registered trademark of Cytiva

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
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