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Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, medium

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About This Item

CAS-Nummer:
9004-54-0
MDL-Nummer:
MFCD00132771
UNSPSC-Code:
23151817
NACRES:
NA.52

Qualität

DNA grade
for molecular biology

Produktlinie

BioReagent

Form

beads

Quellen

1 g swells to 9-11 mL

Perlendurchmesser

50-150 μm

Porengröße

exclusion limit (20 bp dsDNA; (rA)20)

pH-Wert

2-10

Fremdaktivität

DNase, RNase, none detected

Lagertemp.

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChIKey

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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Allgemeine Beschreibung

Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Anwendung

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.
Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.

Leistungsmerkmale und Vorteile

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Rechtliche Hinweise

Sephadex is a registered trademark of Cytiva

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Sigma-Aldrich

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