This product can be dissolved in cold deionized water up to 1000 units per mL. The units per mg are batch-dependent and can be found in the Certificate of Analysis.
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P3026
Pektolyase aus Aspergillus japonicus
lyophilized powder, ≥0.3 units/mg solid
Synonym(e):
Pectolytic enzyme
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About This Item
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Empfohlene Produkte
Biologische Quelle
Aspergillus sp. (A. japonicus)
Form
lyophilized powder
Spezifische Aktivität
≥0.3 units/mg solid
Mol-Gew.
50 kDa
Grünere Alternativprodukt-Eigenschaften
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Grünere Alternativprodukt-Kategorie
, Enabling
Lagertemp.
2-8°C
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Allgemeine Beschreibung
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.[1]
Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.[1]
Anwendung
Zur Herstellung von Pflanzenprotoplast für den Zellwandabbau vor der organellen Isolation.
Pectolyase from Aspergillus japonicus has been used:
- in the lysis of chlorella cells for protoplast generation[2]
- as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation[3]
- to induce reorganization of Arabidopsis root cap cell architecture[4]
Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis[5].
Biochem./physiol. Wirkung
Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora.
Sonstige Hinweise
Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
Für weitere Informationen siehe enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Einheitendefinition
1 U setzt 1.0 μmol Galacturonsäure aus Polygalacturonsäure pro Minute frei bei pH 5.5 und 25 °C.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Susann Hesse et al.
Frontiers in plant science, 10, 773-773 (2019-07-10)
During prophase I a meiosis-specific proteinaceous tripartite structure, the synaptonemal complex (SC), forms a scaffold to connect homologous chromosomes along their lengths. This process, called synapsis, is required in most organisms to promote recombination between homologs facilitating genetic variability and
Identification of sporopollenin as the outer layer of cell wall in microalga Chlorella protothecoides
He X, et al.
Frontiers in Microbiology, 7, 1047-1047 (2016)
P J De Lange et al.
Annals of botany, 101(6), 881-899 (2007-12-07)
Crassula hunua and C. ruamahanga have been taxonomically controversial. Here their distinctiveness is assessed so that their taxonomic and conservation status can be clarified. Populations of these two species were analysed using morphological, chromosomal and DNA sequence data. It proved
Protocol for the preparation of Arabidopsis meiotic chromosome spreads and fluorescent in situ hybridization
Bolanos-Villegas P, et al.
The Plant Journal, 4, e1102-e1102 (2014)
Pectic enzymes in pectolyase: separation, characterization, and induction of ethylene in fruits
Baldwin EA and Pressey R
Plant Physiology, 90(1), 191-196 (1989)
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How to dissolve the lyophilized powder of P3026 coded pectolyase powder? and how to prepare 0.8% solution of it?
1 answer-
Helpful?
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Hello! I want to ask how much cellulase does the pectolyase (P3026) contains? It does not mention any contamination of other enzymes in the product description, but I want to know if there is any potential contamination involved.
1 answer-
This product is not tested for the presence of contaminating enzymes. The primary components are endo-polygalacturonase and endo-pectin lyase. A brief search of the literature shows that in protoplast isolation, cellulase is often added to the digest, in addition to pectolyase.
Helpful?
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Hello, I wanted to ask if this enzyme is equivalent to pectolyase Y-23, as the activity values are expressed differently.
1 answer-
Yes, this product is equivalent to pectolyase Y-23.
Helpful?
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