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Merck

N0252

Sigma-Aldrich

4-Nitrophenyldecanoat

lipase substrate, ≥98% (TLC), powder

Synonym(e):

4-Nitrophenyl-caprat, Decansäure-4-nitrophenylester

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About This Item

Empirische Formel (Hill-System):
C16H23NO4
CAS-Nummer:
Molekulargewicht:
293.36
Beilstein:
1915230
MDL-Nummer:
UNSPSC-Code:
12352204
PubChem Substanz-ID:
NACRES:
NA.83

product name

4-Nitrophenyldecanoat, lipase substrate

Assay

≥98% (TLC)

Form

powder

Löslichkeit

chloroform: soluble 100 mg/mL, colorless to faintly yellow

Lagertemp.

−20°C

SMILES String

CCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C16H23NO4/c1-2-3-4-5-6-7-8-9-16(18)21-15-12-10-14(11-13-15)17(19)20/h10-13H,2-9H2,1H3

InChIKey

RRNKCSIEGPOIKI-UHFFFAOYSA-N

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Anwendung

4-Nitrophenyl decanoate has been used as a substrate for assessing the lipase activity spectrophotometrically. It has also been used as a substrate for estimating the carboxylesterases activity.
4-Nitrophenyl decanoate has been used as a substrate to assess the carboxylesterase production by recombinant Penicillium griseoroseum T55 strain. It has also been used for general esterase activity assay of the hepatopancreas extract.

Biochem./physiol. Wirkung

4-Nitrophenyl decanoate is a substrate for lipase enzyme. The extracellular lipase has greater hydrolysis reaction velocity with 4-nitrophenyl decanoate when compared with other 4-nitropheny esters.

Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

P-Sätze

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Die Dokumentenbibliothek aufrufen

Isolation and characterization of an enzyme from the Greenshell? mussel Perna canaliculus that hydrolyses pectenotoxins and esters of okadaic acid
MacKenzie LA, et al.
Toxicon, 60(3), 406-419 (2012)
V M Gumerov et al.
Prikladnaia biokhimiia i mikrobiologiia, 48(4), 376-382 (2012-10-06)
As a result of sequencing the genome of the termophilic alkali-tolerant lipolytic bacterium Thermosyntropha lipolytica, the gene encoding a lipase secreted into the medium was identified. The recombinant enzyme was expressed in Escherichia coli. It was isolated, purified, and functionally
Kamila Dmochowska et al.
Journal of insect science (Online), 13, 77-77 (2013-11-14)
Bees of the genus Osmia are being used in crop pollination at an increasing rate. However, a short life expectancy of adult individuals limits the feasibility of their use. Cocoons of the red mason bee, Osmia rufa L. (Hymenoptera: Megachilidae)
Janaina Marques de Almeida et al.
Scientific reports, 8(1), 10000-10000 (2018-07-04)
We determined the effect of the His-tag on the structure, activity, stability and immobilization of LipC12, a highly active lipase from a metagenomic library. We purified LipC12 with a N-terminal His-tag and then removed the tag using tobacco etch virus
Methods in Molecular biology: Platelets and Megakaryocytes, volume 1, functional assays, 27-27 (2012)

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