Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
(NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
• Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
• While gently stirring the water, add 100 ml of 10X medium.
• To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
• While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
• Bring medium to final volume with additional tissue culture grade water.
• It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
• Aseptically dispense into sterile containers. Store refrigerated at 2-8° C
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M9288
Minimum Essential Medium Eagle
With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x
Synonym(e):
MEM
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100 ML
CHF 29.90
About This Item
UNSPSC-Code:
12352207
NACRES:
NA.75
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Produktbezeichnung
Minimum Essential Medium Eagle, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Qualitätsniveau
Sterilität
sterile-filtered
Form
liquid
Konzentration
10 ×
Methode(n)
cell culture | mammalian: suitable
Verunreinigungen
endotoxin, tested
Komponenten
NaHCO3: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
HEPES: no
sodium pyruvate: no
phenol red: yes
Versandbedingung
ambient
Lagertemp.
2-8°C
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Allgemeine Beschreibung
Minimum Essential Medium (MEM), one of the most used synthetic cell culture media, is developed by Harry Eagle. It is used to cultivate various types of cells grown in monolayers.
Anwendung
Minimum Essential Medium Eagle has been used as a transport media to maintain pig liver cells.[1]
Rekonstituierung
Supplement with 0.292 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Artikel
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
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If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?
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