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Merck

L5886

Sigma-Aldrich

Lipopolysaccharide aus Salmonella abortus equi

purified by phenol extraction

Synonym(e):

LPS

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352201
NACRES:
NA.25
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Biologische Quelle

Salmonella enterica (Serotype abortus equi)

Qualitätsniveau

Form

lyophilized powder

Aufgereinigt durch

phenol extraction

Verunreinigungen

<3% Protein (Lowry)

Farbe

white to faint gray

Löslichkeit

water: soluble

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

This product is phenol extracted from Salmonella abortus equi. The source strain is ATCC 9842. Lipopolysaccharide from Salmonella abortus equi stimulates human mononuclear cells to produce tumor necrosis factor. This LPS is also mitogenic for frog and mouse lymphocytes.

Anwendung

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem./physiol. Wirkung

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Angaben zur Herstellung

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Sonstige Hinweise

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Ähnliches Produkt

Piktogramme

Skull and crossbones

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Acute Tox. 2 Oral

Lagerklassenschlüssel

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Artikel

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

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