GE11-0034-94

HiTrap^® MabSelect SuRe^™

Cytiva 11-0034-94, pack of 1 × 5 mL

• Synonym(e): HiTrap MabSelect SuRe, MabSelect SuRe Column
• UNSPSC-Code: 23151816
• NACRES: NA.56

Eigenschaften

• ligand: alkali-stabilized protein A-derived (E. coli)
• Verpackung: pack of 1 × 5 mL
• Hersteller/Markenname: Cytiva 11-0034-94
• Lagerbedingungen: (20% Ehtanol); (20% Ethanol)
• Parameter: <20 mL/min flow rate; 42 psi
• Bettgröße: 16 mm × 25 mm
• Bettvolumen: 5 mL
• Säule ID: 16 mm
• Matrix: highly cross-linked agarose
• Durchschnittliche Teilgröße: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-13.7
• working range: 3-12
• Kapazität: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• Eignung: suitable for bioprocess medium
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap^® MabSelect^™ SuRe^™ columns are prepacked with MabSelect^™ SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).

HiTrap^® MabSelect^™ columns are prepacked with MabSelect^™ a bioprocess medium for capuring of Mabs from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Leistungsmerkmale und Vorteile

• Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
• Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap^® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

MabSelect SuRe is a trademark of Cytiva

SuRe is a trademark of Cytiva

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Lagerklassenschlüssel: 3 - Flammable liquids