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G8255

Sigma-Aldrich

Glutam-Brenztraubentransaminase aus Schweineherz

lyophilized powder, ≥75 units/mg protein

Synonym(e):

L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT

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About This Item

CAS-Nummer:
9000-86-6
EC-Nummer:
2.6.1.2 (BRENDA, IUBMB)
Beilstein:
3562140
EG-Nummer:
232-561-4
MDL-Nummer:
MFCD00131192
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

Porcine heart

Form

lyophilized powder

Spezifische Aktivität

≥75 units/mg protein

Zusammensetzung

Protein, 75-95%

Methode(n)

cell culture | mammalian: suitable

Farbe

light yellow to yellow

Fremdaktivität

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.1%

Lagertemp.

−20°C

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Allgemeine Beschreibung

Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.

Research area: Cell Signaling

Anwendung

Glutamic-Pyruvic Transaminase from porcine heart has been used:
  • in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate
  • to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells
  • in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies

Biochem./physiol. Wirkung

Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate.Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Einheitendefinition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Physikalische Form

Lyophilized powder containing citrate and acetate

Lagerung und Haltbarkeit

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Hinweis zur Analyse

Protein determined by biuret

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Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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Journal of the American Chemical Society, 129(39), 12011-12018 (2007-09-06)
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A simple and sensitive detection of glutamic-pyruvic transaminase activity based on fluorescence quenching of bovine serum albumin
Chen M, et al.
Royal Society of Chemistry Advances, 5, 103557-103562 (2015)
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Faqi A S
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Journal of biochemical and biophysical methods, 64(3), 167-181 (2005-08-23)
The surface expression of G protein-coupled receptors is regulated by internalization. For many receptors, a constitutive level of internalization in the absence of agonist has been reported. The constitutive internalization of metabotropic glutamate receptor 1a (mGluR1a) has been described, but
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