SAE0049

L-Lactat-Dehydrogenase (LDHA)

from human, recombinant, expressed in E. coli, aqueous solution

• Synonym(e): L-Lactat-Dehydrogenase, rekombinant aus E. coli, (S)-Lactat: NAD⁺ Oxidoreduktase, Milchsäuredehydrogenase, rekombinant
• CAS-Nummer: 9001-60-9
• EC-Nummer: 1.1.1.27 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131466
• UNSPSC-Code: 12352200
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Form: aqueous solution
• Lagerbedingungen: (Keep container tightly closed in a dry and well-ventilated place)
• Farbe: colorless
• UniProt-Hinterlegungsnummer: K1T0A2
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... LDHA(3939)

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signaling
The gene LDHA (L-lactate dehydrogenase A chain) is mapped to human chromosome 11p15.[1] It is a subunit of lactate dehydrogenase.[2]In particular, lactic dehydrogenase A (LDHA) is mainly found in skeletal muscle, and for that reason is known as the M subunit. This recombinant form of LDHA has a C-terminal histidine-tag.

Anwendung

L-Lactate Dehydrogenase (LDHA) has been used in in vitro phosphoglycerate mutase 1 (PGAM1) inhibitors screening assay.[3] It has also been used in a colorimetric assay for determining lactate concentration in conditioned media.[4]

Biochem./physiol. Wirkung

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.[5]  L-lactate dehydrogenase A chain (LDHA), an enzyme involved in pyruvate metabolism,[6] LDH is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers.[6][7] LDHA regulates the microenvironment of developing tumors by the hypoxia-inducible factor (HIF)-signaling pathway.[7] LDHA aids in the NAD⁺ regeneration during the β-oxidation of fatty acid.[8] LDHA (L-lactate dehydrogenase A chain) is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. In cancer cells, HIF-1a (hypoxia-inducible factor) induces the expression of LDHA, which helps in maintaining glycolysis in cells.[2]

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.

Einheitendefinition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physikalische Form

Buffered aqueous solution with Hepes (pH 7.5), NaCl and glycerol.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable