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Merck

C5499

Sigma-Aldrich

Cytochrome c Oxidase from bovine heart

greener alternative

5 mg protein/mL

Synonym(e):

EC 1.9.3.1, Ferrocytochrome-c:oxygen oxidoreductase, Complex IV

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About This Item

CAS-Nummer:
EC-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.32

Biologische Quelle

bovine heart

Qualitätsniveau

Form

buffered aqueous solution

Spezifische Aktivität

≥20 units/mg protein

Mol-Gew.

300-350 kDa by gel filtration

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Konzentration

5 mg protein/mL

Grünere Alternativprodukt-Kategorie

Versandbedingung

dry ice

Lagertemp.

−20°C

Allgemeine Beschreibung

Research area: Cell cycle

Cytochrome c oxidase contains 13 subunits, encoded by mitochondrial genes and nuclear genome. It is comprised of two heme groups (Fea and Fea3) and two copper centers, CuA and CuB.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles.

Anwendung

Cytochrome c Oxidase (CcO) from bovine heart has been used:
  • to study the effect of oxidized glutathione (GSSG) on the number of total free cysteines
  • in a histochemical assay to measure CcO activity in tissue sections
  • to study the redox reactions of cytochrome c in isolated mitochondria exposed to blue or red lasers using resonance Raman spectroscopy

Biochem./physiol. Wirkung

Cytochrome c oxidase is the principal terminal oxidase of high oxygen affinity in the aerobic metabolism of all animals, plants, yeasts and some bacteria. It is present in the mitochondria of the more highly developed cells and in the cytoplasmic membrane of bacteria. Cytochrome c oxidase catalyses the electron transfer from cytochrome c to O2. This electron-transfer process produces a proton gradient across the membrane, which in turn drives the production of ATP. This enzyme is unique in providing energy for the cell bycoupling the electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Einheitendefinition

One unit of cytochrome c oxidase will oxidize 1.0 μmole of ferrocytochrome c per min. at 25 °C at pH 6.0.

Physikalische Form

Supplied as a solution in 25 mM Tris-HCl buffer, pH 7.8, 5 mM EDTA, and 39 mM n-dodecyl β·-D-maltoside.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Cytochrome c Oxidase Dysfunction in Oxidative Stress
Srinivasan S & Avadhani NG
Free Radical Biology & Medicine (2013)
Reply to Giamogante et al.: The effect of low cyanide on O2 consumption is best observed in physiological, rather than reductionist, systems.
Karim Zuhra et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(39) (2021-09-22)
Sze Kiat Sim et al.
Turkish neurosurgery, 25(4), 617-624 (2015-08-06)
This study evaluated the neuroprotective effect of intrathecally infused paclitaxel in the prevention of motoneuron death and mitochondrial dysfunction following brachial plexus avulsion injury. Brachial root avulsion injury was induced in Sprague-Dawley rats. The Paclitaxel treatment group (n = 32)
Claudia R Oliva et al.
Oncotarget, 8(23), 37568-37583 (2017-04-30)
Patients with glioblastoma have one of the lowest overall survival rates among patients with cancer. Standard of care for patients with glioblastoma includes temozolomide and radiation therapy, yet 30% of patients do not respond to these treatments and nearly all
Zong-Heng Wang et al.
eLife, 8 (2019-10-16)
Oogenesis features an enormous increase in mitochondrial mass and mtDNA copy number, which are required to furnish mature eggs with an adequate supply of mitochondria and to curb the transmission of deleterious mtDNA variants. Quiescent in dividing germ cells, mtDNA

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