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D5637
3,3′-Diaminobenzidin -tetrahydrochlorid Hydrat
≥96%
Synonym(e):
(1,1′-Biphenyl)-3,3′, 4,4′-tetramintetrahydrochlorid-Dihydrat
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 g | Warenkorb auf Verfügbarkeit prüfen | CHF 63.80 |
5 g | Warenkorb auf Verfügbarkeit prüfen | CHF 221.00 |
10 g | Warenkorb auf Verfügbarkeit prüfen | CHF 405.00 |
25 g | Warenkorb auf Verfügbarkeit prüfen | CHF 877.00 |
50 g | Warenkorb auf Verfügbarkeit prüfen | CHF 1’260.00 |
Über diesen Artikel
Lineare Formel:
C12H14N4 · 4HCl · xH2O
CAS-Nummer:
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
231-018-9
MDL number:
Technischer Dienst
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Unterstützung erhaltenQuality Segment
assay
≥96%
form
powder, crystals or chunks
technique(s)
titration: 96-104% (with NaOH)
color
white to light brown, and Faint Red and Faint Grey to Light Grey
mp
280 °C
solubility
H2O: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
InChI key
DXWSCXIZIHILNP-UHFFFAOYSA-N
General description
3,3′-Diaminobenzidin-Tetrahydrochlorid ist ein Chromogen, das häufig in der Immunhistochemie und anderen Färbetechniken zur Visualisierung von Antigenen oder Antikörpern in biologischen Proben verwendet wird. DAB-HCl dient als immunhistologisches Färbemittel zur Visualisierung der Peroxidase-Aktivität in Zellen. Die DAB-Oxidation kann mit einem Spektrofotometer gemessen werden.
Application
3,3′-Diaminobenzidintetrahydrochloridhydrat eignet sich für immunhistologische Färbungsverfahren, Western Blot und FISH (Fluoreszenz-in-situ-Hybridisierung). DAB wird zur Untersuchung der Epithelzellapoptose im Falle von früh auftretendem Katarakt und in immunhistochemischen Protokollen zur autosomal-dominanten polyzystischen Niere (PKD, polycystic kidney disease) eingesetzt.
Biochem/physiol Actions
In der Peroxidasereaktion dient DAB als Wasserstoffdonator in Gegenwart von Peroxid. Das oxidierte DAB bildet einen unlöslichen braunen Komplex.
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Dieser Artikel | |||
|---|---|---|---|
| technique(s) titration: 96-104% (with NaOH) | technique(s) - | technique(s) UV/Vis spectroscopy: suitable | technique(s) - |
| color white to light brown, and Faint Red and Faint Grey to Light Grey | color off-white to faint red, to beige | color - | color - |
| form powder, crystals or chunks | form powder | form - | form powder |
| solubility H2O: 50 mg/mL | solubility water: 50 mg/mL, clear to slightly hazy | solubility - | solubility acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| storage temp. −20°C | storage temp. room temp | storage temp. - | storage temp. 2-8°C |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
Lagerklasse
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
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Do you have a staining protocol for Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 answer-
Dissolve 10 mg in 15 mL of Tris buffered saline, pH 7.6 (Tris-buffered saline tablet, Sigma Product No. T5030 may be used). Add 12 uL of fresh 30% hydrogen peroxide (Sigma Product No. H1009) prior to use. Solution may be filtered through a 0.2 micron filter immediately prior to use if necessary. DAB and hydrogen peroxide concentrations may be adjusted to suit individual applications. The stain can be enhanced with Ni. Make up 0.3% (w/v) NiCl2 in water. Add 1 mL of the NiCl2 stock to 9 mL DAB solution.
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What is the solution stability of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 answer-
In our experience, solutions of DAB should be made immediately prior to use for best results. If and only if necessary, solutions can be kept in the dark and refrigerated for overnight storage. Based on our Immunology Department’s experience, storing the solution overnight did not give good reproducibility. However, the literature indicates that it is possible to store solutions of DAB at -20°C. DAB dissolved in Tris buffered saline (pH 7.6) at 0.6 mg/ml was reported to be stable for several weeks at 4°C.
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How is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), shipped and stored?
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The product is shipped at ambient temperature. However, it is stored at -20 degrees C for long term storage.
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Can the solutions of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), be filtered?
1 answer-
DAB solution may be filtered through a 0.2 micron filter immediately prior to use.
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What is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), soluble in?
1 answer-
DAB HCl is soluble in water at 50 mg/ml yielding a clear to slightly hazy solution. For consistency, it is important to allow DAB to come to room temperature before making solutions. Water quality is extremely important, so only high quality deionized or distilled water should be used to make DAB solutions.
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What is the shelf life of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 answer-
This product has a shelf life of 3 years.
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