C0662

6-Carboxyfluorescein

~97% purity (HPLC), powder

• Synonym(e): 6-FAM, FLUOS
• Empirische Formel (Hill-System): C₂₁H₁₂O₇
• CAS-Nummer: 3301-79-9
• Molekulargewicht: 376.32
• Beilstein: 54341
• MDL-Nummer: MFCD00036873
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24892266
• NACRES: NA.47

Eigenschaften

• Produktbezeichnung: 6-Carboxyfluorescein, ≥96% (HPLC)
• Qualitätsniveau: 200
• Assay: ≥96% (HPLC)
• Form: powder
• Methode(n): titration: suitable
• Farbe: dark yellow to dark orange
• mp (Schmelzpunkt): >300 °C (lit.)
• Löslichkeit: 0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange
• λmax: 492 nm at 0.1 N in NaOH
• Fluoreszenz: λ_ex 492 nm; λ_em 517 nm (pH 8.0)
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: 2-8°C
• SMILES String: OC(=O)c1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
• InChI: 1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChIKey: BZTDTCNHAFUJOG-UHFFFAOYSA-N

Beschreibung

Anwendung

Fluoreszierender Farbstoff.

6-Carboxyfluorescein has been used to check the plasma membrane integrity in sperm cells.[1]

Biochem./physiol. Wirkung

6-Carboxyfluorescein diacetate is a membrane permeable compound. It can be hydrolysed by intracellular esterases to form a membrane impermeable bright green fluorescent molecule, 6-carboxyfluorescein.[2]

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves