46950

Fluoreszein-5(6)-isothiocyanat

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

• Synonym(e): FITC
• Empirische Formel (Hill-System): C₂₁H₁₁NO₅S
• CAS-Nummer: 27072-45-3
• Molekulargewicht: 389.38
• UNSPSC Code: 12171500
• NACRES: NA.32
• PubChem Substance ID: 329757273
• EC Number: 248-207-7
• Beilstein/REAXYS Number: 1407295
• MDL number: MFCD00005063

Eigenschaften

• Quality Segment: 100
• product line: BioReagent
• assay: ≥90% (HPLC)
• form: powder
• mol wt: 389.38 g/mol
• color: orange to dark orange, yellow to dark yellow
• solubility: acetone: 0.1 g/10 mL, clear to very slightly hazy
• fluorescence: λ_ex 492 nm; λ_em 518 nm in 0.1 M phosphate pH 8.0
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Beschreibung

General description

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.

Application

Für die Fluoreszenzmarkierung von Proteinen (z.B. Immunofluoreszenz); H Protein-Labeling für die Gelchromatographie.

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves
• pictograms: GHS07
• signalword: Warning
• hcodes: H302,H315,H317
• pcodes: P261 - P264 - P272 - P280 - P301 + P312 - P302 + P352
• Hazard Classifications: Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B