21878

5(6)-Carboxyfluoreszein-N-hydroxysuccinimidester

BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

• Synonym(e): 5(6)-FAM OSu, FLUOS
• Empirische Formel (Hill-System): C₂₅H₁₅NO₉
• CAS-Nummer: 117548-22-8
• Molekulargewicht: 473.39
• NACRES: NA.32
• PubChem Substance ID: 57647947
• UNSPSC Code: 12352108
• MDL number: MFCD04119713

Eigenschaften

• Quality Segment: 200
• product line: BioReagent
• assay: ≥80% (HPLC)
• form: powder
• solubility: DMF: soluble, DMSO: soluble
• fluorescence: λ_ex 492 nm; λ_em 517 nm in DMF
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: OC(=O)c1ccc(cc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O.OC(=O)c6cccc(C(=O)ON7C(=O)CCC7=O)c6C8=C9C=CC(=O)C=C9Oc%10cc(O)ccc8%10
• InChI: 1S/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)
• InChI key: YVTPVJDECOUTJW-UHFFFAOYSA-N

Beschreibung

Application

Fluoreszenzlabel für primäre und sekundäre Amin-Gruppen von Proteinen und anderen Biomolekülen.

Wurde verwendet, um fluoreszierende Tubulin-Derivate herzustellen, sowie für die Herstellung eines Phenytoin-Tracers für die Bestimmung dieses Wirkstoffs in Serum und Plasma mittels Fluoreszenz-Polarisations-Immunoassay (FPIA)

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)