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Sigma-Aldrich

1,3,5-Triphenyl-tetrazolium-formazan

≥90% (UV)

Synonym(e):

TTC-formazan

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About This Item

Empirische Formel (Hill-System):
C19H16N4
CAS-Nummer:
531-52-2
Molekulargewicht:
300.36
Beilstein:
753057
EG-Nummer:
208-509-1
MDL-Nummer:
MFCD00020647
UNSPSC-Code:
12352204
PubChem Substanz-ID:
57653521
NACRES:
NA.83

Assay

≥90% (UV)

Form

powder

Löslichkeit

dioxane: 50 mg/mL, clear

SMILES String

N(\N=C(/N=N/c1ccccc1)c2ccccc2)c3ccccc3

InChI

1S/C19H16N4/c1-4-10-16(11-5-1)19(22-20-17-12-6-2-7-13-17)23-21-18-14-8-3-9-15-18/h1-15,20H/b22-19-,23-21+

InChIKey

BEIHVSJTPTXQGB-YMGPPEANSA-N

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Allgemeine Beschreibung

1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible. TTC formazan is brightly colored and can be quantified using a spectrophotometer.

Anwendung

1,3,5-Triphenyltetrazolium formazan has been used:
  • for the quantification of 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity in bacterial cells
  • as a standard to study the optical density of formazan produced by TTC-treated milled seeds
  • to stain heart tissue for infarct size assessment or undergo perfusion fixation to allow morphometric studies in addition to histology and immunohistochemistry

Sonstige Hinweise

Review: Tetrazolium salts and formazans

Piktogramme

Exclamation markEnvironment
GHS07,GHS09

Signalwort

Warning

H-Sätze

H302,H400

Gefahreneinstufungen

Acute Tox. 4 Oral - Aquatic Acute 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Se-Chan Kim et al.
Journal of visualized experiments : JoVE, (65), e3896-e3896 (2012-08-01)
Surgical trauma by thoracotomy in open-chest models of coronary ligation induces an immune response which modifies different mechanisms involved in ischemia and reperfusion. Immune response includes cytokine expression and release or secretion of endogenous ligands of innate immune receptors. Activation
Quantification of triphenyl-2H-tetrazoliumchloride reduction activity in bacterial cells
Defez R, et al.
Bio-protocol, 7(2), e2115-e2115 (2017)
Laura Lopez Del Egido et al.
Frontiers in plant science, 8, 747-747 (2017-06-01)
A comparative analysis was carried out of published methods to assess seed viability using 2,3,5-triphenyltetrazolium chloride (TTC) based assays of seed batches. The tests were carried out on seeds of barley (Hordeum vulgare cv. Optic) as a model. We established
Roberto Defez et al.
Bio-protocol, 7(2), e2115-e2115 (2017-01-20)
This protocol describes the use of the 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt to evaluate the cell redox potential of rhizobia cells. The production of brightly colored and insoluble 1,3,5-Triphenyltetrazolium formazan arising from TTC reduction is irreversible and can be easily quantified
F.P. Altman
Progress in Histochemistry and Cytochemistry, 9(3), 51-51 (1976)

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