W0625

Wright-Färbung

≤4% purity (HPLC), powder

• Synonym(e): Eosin-Methylenblau nach Wright
• CAS-Nummer: 68988-92-1
• MDL-Nummer: MFCD00082143
• UNSPSC-Code: 12171500
• NACRES: NA.47

Eigenschaften

• product name: Wright-Färbung, suitable for blood stain
• Assay: ≤4% (HPLC)
• Form: powder
• Konzentration: ≤4% (HPLC)
• Farbe: dark green
• Löslichkeit: methanol: 10 mg/10 mL
• Eignung: suitable for blood stain
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: room temp
• InChI: 1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1
• InChIKey: AXIKDPDWFVPGOD-UHFFFAOYSA-O

Beschreibung

Allgemeine Beschreibung

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Anwendung

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

• It is also used for G-banding and helps in the analysis of poorly spread metaphases.
• It has been used to study the cytogenetics of human hematopoietic cells.
• It has also been employed in the karyotyping of human embryonic stem cells.
• It is also used in the staining of chromosomes.
• It is used for the exudate analysis of white blood cell concentration.

Verwendung zur Färbung von Blutzellen

Leistungsmerkmale und Vorteile

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

Angaben zur Herstellung

Hergestellt unter der Verwendung von polychromen (oxidiertem) Methylenblau und Eosin Y.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H302,H319
• P-Sätze: P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313
• Gefahreneinstufungen: Acute Tox. 4 Oral - Eye Irrit. 2
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves