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Merck

WG16

Sigma-Aldrich

Wright-Giemsa Stain, Modified

Wright-Giemsa Stain, Modified
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About This Item

MDL-Nummer:
UNSPSC-Code:
41116121
eCl@ss:
42039003
NACRES:
NA.47
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Form

solution

Qualitätsniveau

Haltbarkeit

Expiry date on the label.

IVD

for in vitro diagnostic use

Konzentration

0.4 % (w/v) in methanol (buffered at pH 6.8)

pH-Wert

6.7-6.9

Anwendung(en)

hematology
histology

Lagertemp.

room temp

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Anwendung

When blood films are stained using Wright-Giemsa Stain, the white blood cell nucleus and cytoplasm take on the characteristic blue or pink coloration. The combination of purified eosin and thiazine dyes in the product eliminates inconsistent staining and yields reproducible chromogenic responses.

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 1

Zielorgane

Eyes,Central nervous system

Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 2

Flammpunkt (°F)

51.8 °F - closed cup

Flammpunkt (°C)

11 °C - closed cup

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Questions

  1. What is the preservation process for slides stained with the Wright-Giemsa stain if they cannot be viewed immediately?

    1 answer
    1. the Wright Giemsa Modified solution is intended for staining peripheral blood or bone marrow smears, without mention of staining tissue sections. If staining smears, simply complete the staining procedure as directed in the IFU, then store the dried slides in a slide folder or box without coverslipping, as coverslipping is not recommended and is not a common practice in hospital settings.

      For long-term storage, slides are typically stored in slide file cabinets, and oil immersion is usually not required. However, if slides need to be viewed under oil immersion, a coverslip can be placed atop the stained slide, and once viewed, the coverslip can be removed and discarded. Any excess oil can be absorbed using a Kimwipe or tissue paper without wiping, as wiping may remove stained cells from the slide.

      Although no formal studies have been conducted to determine the longevity of stained slides, it is expected that the staining should be adequate for several weeks or months. For longer retention, it is suggested to take pictures of several fields from the slides for archival purposes.

      Helpful?

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