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I8377

Sigma-Aldrich

Jodnitrotetrazoliumchlorid

Used in colorimetric assays.

Synonym(e):

2-(4-Iodphenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchlorid, p-Iodnitrotetrazolium violett, INT

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About This Item

Empirische Formel (Hill-System):
C19H13ClIN5O2
CAS-Nummer:
146-68-9
Molekulargewicht:
505.70
Beilstein:
4093224
EG-Nummer:
205-676-2
MDL-Nummer:
MFCD00011961
UNSPSC-Code:
12171500
PubChem Substanz-ID:
24896138
NACRES:
NA.47

Qualitätsniveau

200

Assay

≥98% (TLC)

Form

powder

mp (Schmelzpunkt)

240 °C (dec.) (lit.)

Löslichkeit

H2O: 4 mg/mL

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

2-8°C

SMILES String

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

InChIKey

JORABGDXCIBAFL-UHFFFAOYSA-M

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Anwendung

Iodnitrotetrazoliumchlorid wird für die Färbung von Zellen verwendet. Es wird als Kopplungsmittel in D-Arabinitol-Enzym-Assays eingesetzt.
Elektronenakzeptor für die kolorimetrische Untersuchung verschiedener Dehydrogenasen

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Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

FlameExclamation mark
GHS02,GHS07

Signalwort

Danger

H-Sätze

H228,H335

Gefahreneinstufungen

Flam. Sol. 1 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

4.1B - Flammable solid hazardous materials

WGK

WGK 3

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Analysenzertifikate (COA)

Lot/Batch Number
0000284108
0000310220
SLCQ5874
SLCL0301
SLBZ3933

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Journal of biochemical and biophysical methods, 68(2), 101-111 (2006-06-03)
A sensitive spectrophotometric assay for determining mitochondrial malate dehydrogenase activity is described. The assay measures NADH production by coupling it to the reduction of 2-(p-iodophenyl)-3(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT). Via an intermediate electron carrier, either phenazine methosulfate or lipoamide dehydrogenase, INT
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