This product does not require activation. Note that this material has not been tested for use in cell culture. Suitability would have to be determined by the end user.
CC4000
Urokinase-type Plasminogen Activator
Synonym(e):
uPA
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Empfohlene Produkte
Biologische Quelle
human
Qualitätsniveau
Assay
>98% (SDS-PAGE)
Form
solid
Hersteller/Markenname
Chemicon®
Methode(n)
activity assay: suitable
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Angaben zum Gen
human ... PLAU(5328)
Allgemeine Beschreibung
A thrombolytic agent that converts plasminogen to plasmin. A mixture of high molecular weight and low molecular weight urokinase.
Product Source: Prepared from urine of healthy individuals
Physikalische Form
Lyophilized from 200 mM manitol, 100 mM NaCl, 50 mM Tris-HCl, pH 7.5 and 0.1 % PEG.
Lagerung und Haltbarkeit
Reconstitute using 200-400 μL dH2O. Store at -20°C. Following reconstitution, aliquot and freeze (-70°C). This product is stable for 2 year as supplied. Stock solutions are stable for up to 6 months at -70°C.
Hinweis zur Analyse
High molecular weight urokinase (54 kD): 40%.Low molecular weight urokinase (33 kD): 50%.Transitory forms of urokinase: 10%
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Kunden haben sich ebenfalls angesehen
Thrombolytic therapy: a comparison between urokinase and streptokinase. From a national cooperative study.
W R Bell
Seminars in thrombosis and hemostasis, 2(1), 1-13 (1975-07-01)
Assay methods and standard preparations for plasmin, plasminogen and urokinase in purified systems, 1967-1968.
Johnson, A J, et al.
Thrombosis et Diathesis Haemorrhagica, 21, 259-272 (1969)
Sean T Zuckerman et al.
Biomaterials, 30(23-24), 3825-3833 (2009-05-16)
Mass spectrometry is a powerful proteomic tool enabling researchers to survey the global proteome of a cell. This technique has only recently been employed to investigate cell-material interactions. We had previously identified material scarcity and limited adherent cells as challenges
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Hello, I am interested in adding uPA to the medium of my cell culture of human pluripotent stem cell. Is product number CC4000 suitabl and ready to add to the medium, or does the protein needs activation before usage?
1 answer-
Helpful?
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Is CC4000, Urokinase-type Plasminogen Activator is in its active conformation and ready to be used in protease activity assays, or it needs to be activated before use?
Is CC4000, Urokinase-type Plasminogen Activator is in its active conformation and ready to be used in protease activity assays, or it needs to be activated before use?
1 answer-
This product is a combination of active (low molecular weight) and inactive (high molecular weight) urokinase. The composition of the CC4000 product is as follows:
Low molecular weight urokinase (33 kD) 50%
High molecular weight urokinase (54 kD) 40%
Transient forms of urokinase 10%
Activation prior to use is not required.
uPA is produced as an inactive form called pro-uPA which is 54KDa. Pro-uPA is cleaved by proteases to produce a low molecular weight active form that is 33 kDa. This cleavage by plasmin occurs in two steps. The transitory form is the result of the first cleavage step. The second cleavage of this transient form results in the active 33 kDa uPA. The link below may provide additional helpful information:Helpful?
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