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14-448-M

Sigma-Aldrich

Cdk2/Cyclin A Protein, active, 10 µg

Active, C-terminal His6-tagged human full length Cdk2 & N-terminal GST-tagged human full length Cyclin A, for use in Kinase Assays.

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About This Item

UNSPSC-Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

Biologische Quelle

human

Qualitätsniveau

Rekombinant

expressed in baculovirus infected Sf21 cells

Produktlinie

Upstate®

Spezifische Aktivität

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

Mol-Gew.

Mw 35 kDa

Aufgereinigt durch

(The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose)

Speziesreaktivität

human

Hersteller/Markenname

Upstate®

Methode(n)

activity assay: suitable (kinase)

Hinterlegungsnummer

 (FUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. )
NM_001798.2

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Allgemeine Beschreibung

C-terminal His6-tagged human full length cdk2, and N-terminal GST-tagged human full length cyclin A

Biochem./physiol. Wirkung

Protein Target: CDK2/cyclinA
Target Sub-Family: CMGC

Verpackung

Also available in 10 μg size and in bulk
Also available in 250μg size, please inquire for pricing and availability.

Qualität

routinely evaluated by phosphorylation of Histone H1

Zielbeschreibung

CDK2/cyclinA

Verlinkung

Replaces: 14-407

Physikalische Form

The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose

Lagerung und Haltbarkeit

6 months at -70°C

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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