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900622

Sigma-Aldrich

Gelatine-Methacryloyl

gel strength 300 g Bloom, degree of substitution 60%

Synonym(e):

GelMA, Gelatine-Methacrylamid, Gelatine-Methacrylat, GelMa

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About This Item

Lineare Formel:
(C40H59N11O13)n
UNSPSC-Code:
12352202
NACRES:
NA.23

CHF 259.00

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Qualitätsniveau

100

Form

powder

Lagertemp.

2-8°C

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Anwendung

Gelatine-Methacryloyl kann zur Bildung hochvernetzter Hydrogele zur Gewebezüchtung[1] und zum 3D-Druck[2][3][4] eingesetzt werden. Es wird für Endothelzellen-Morphogenese[5], Kardiomyozyten[6], epidermales Gewebe[7], injizierbare Gewebekonstrukte[8], Knochendifferenzierung[9] und Knorpelregeneration[10] verwendet. Gelatine-Methacryloyl wird in Anwendungen zur Arzneimittelverabreichung in Form von Mikrosphären[11] und Hydrogelen[12] erforscht.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
MKCT0116
MKCT0113
MKCT0119
MKCS2673
MKCQ6360

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Xin Zhao et al.
Advanced healthcare materials, 5(1), 108-118 (2015-04-17)
Natural hydrogels are promising scaffolds to engineer epidermis. Currently, natural hydrogels used to support epidermal regeneration are mainly collagen- or gelatin-based, which mimic the natural dermal extracellular matrix but often suffer from insufficient and uncontrollable mechanical and degradation properties. In
Jason W Nichol et al.
Biomaterials, 31(21), 5536-5544 (2010-04-27)
The cellular microenvironment plays an integral role in improving the function of microengineered tissues. Control of the microarchitecture in engineered tissues can be achieved through photopatterning of cell-laden hydrogels. However, despite high pattern fidelity of photopolymerizable hydrogels, many such materials
Anh H Nguyen et al.
Acta biomaterialia, 13, 101-110 (2014-12-03)
Gelatin has been commonly used as a delivery vehicle for various biomolecules for tissue engineering and regenerative medicine applications due to its simple fabrication methods, inherent electrostatic binding properties, and proteolytic degradability. Compared to traditional chemical cross-linking methods, such as
Chaenyung Cha et al.
Biomacromolecules, 15(1), 283-290 (2013-12-19)
Microfabrication technology provides a highly versatile platform for engineering hydrogels used in biomedical applications with high-resolution control and injectability. Herein, we present a strategy of microfluidics-assisted fabrication photo-cross-linkable gelatin microgels, coupled with providing protective silica hydrogel layer on the microgel
Kristel W M Boere et al.
Acta biomaterialia, 10(6), 2602-2611 (2014-03-05)
Hydrogels can provide a suitable environment for tissue formation by embedded cells, which makes them suitable for applications in regenerative medicine. However, hydrogels possess only limited mechanical strength, and must therefore be reinforced for applications in load-bearing conditions. In most
Alle zugehörigen Dokumente anzeigen

Artikel

Photo-Crosslinkable Gelatin Hydrogel: Versatile Materials for (High Resolution) Additive Manufacturing

Discussion of synthetic modifications to gelatin, improving the three-dimensional (3D) print resolution, and resulting material properties.

Three-Dimensional Bioprinting for Tissue and Disease Modeling

Professor Shrike Zhang (Harvard Medical School, USA) discusses advances in 3D-bioprinted tissue models for in vitro drug testing, reviews bioink selections, and provides application examples of 3D bioprinting in tissue model biofabrication.

Protokolle

KAPA SYBR® FAST One-Step qRT-PCR Kits FAQs

Frequently asked questions (FAQs) for KAPA SYBR® FAST One-Step qRT-PCR Kits.

Verwandter Inhalt

Gewebezüchtung

Bei der Gewebezüchtung werden Gewebekulturen aus strukturellen Gerüsten, lebenden Zellen und biologisch aktiven Molekülen hergestellt, indem die Mikroumgebung des Körpers simuliert wird. Ziel ist es, beschädigtes Gewebe zu reparieren oder zu ersetzen.

Tissue Engineering

Tissue engineering fabricates tissues cultures from scaffolds, living cells, and biologically active molecules by simulating the microenvironment of the body to repair or replace damaged tissue.

Questions

1–3 of 3 Questions  

  1. Is this product sterile or not, how to sterilize it?

    1 answer

    1. This product is not sterilized. Sterilization can be achieved by passing a solution through a 0.2 μm PES syringe filter.

      Loessner, D., Meinert, C., Kaemmerer, E. et al. Functionalization, preparation and use of cell-laden gelatin methacryloyl–based hydrogels as modular tissue culture platforms. Nat Protoc 11, 727–746 (2016). https://doi.org/10.1038/nprot.2016.037

      Helpful?

  2. Can you provide guidance on reconstituting the GelMA purchased from Sigma? There is no protocol available online.

    1 answer

    1. There is no protocol available for reconstituting or testing the solubility of the product 900622.
      Here is some information on solubility:
      1.Mix LAP in 3 ml of PBS or cell media at 60°C under stirring to create a 0.5% w/v LAP concentration.
      2.Mix until all LAP is dissolved. Add GelMA to the solution to create a 10% w/v concentration.
      3.Mix at 60°C under stirring until all GelMA is dissolved.

      Helpful?

  3. Sind in dem Produkt Antioxidantien/Inhibitoren wie etwa Topanol A enthalten oder handelt es sich um einen Reinstoff?

    1 answer

    1. The composition of this product is considered proprietary.

      Helpful?

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