G1890

Porcine Gelatin

from porcine skin, Type A, powder, gel strength ~300 g Bloom, suitable for electrophoresis and cell culture

• CAS-Nummer: 9000-70-8
• EG-Nummer: 232-554-6
• MDL-Nummer: MFCD00081638
• UNSPSC-Code: 12352202
• NACRES: NA.75

Eigenschaften

• Produktbezeichnung: Gelatine aus Schweinehaut, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture
• Biologische Quelle: Porcine skin
• Qualitätsniveau: 200
• Sterilität: sterile
• Typ: Type A
• Produktlinie: BioReagent
• Form: powder
• Mol-Gew.: 50—100 kDa
• Verpackung: pkg of 1 kg; pkg of 100 g; pkg of 500 g
• Konzentration: 70—90% Biuret
• Methode(n): cell culture | mammalian: suitable; electrophoresis: suitable
• Oberflächendeckung: 100‑200 μg/cm²
• Löslichkeit: H₂O: soluble 50 mg/mL, clear to hazy, faintly yellow
• Versandbedingung: ambient
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred as type A. Porcine gelatin comprises majorly glycine, proline and hydroxyproline.[1] Gelatin takes up random coil structure after digestion from the triple helical collagen.[2] It differs from type B bovine gelatin at the N-terminal sequence.[3]

Anwendung

Gelatin has been used in many applications. It has been used in coating cell culture to improve attachment of cells,[4][5] being added to PCR to stabilize Taq DNA, as a blocking reagent in western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has been used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications.[6] In the pharmaceutical industry, gelatin can be used as a suspending and encapsulating agent,[7] among other applications. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Leistungsmerkmale und Vorteile

• Sterile gelatin sourced from porcine skin.
• Free from proteases.
• In the form of gelatin powder for convenient and easy handling.
• Versatile for use in cell culture and electrophoresis techniques.
• Provides adequate surface coverage for various applications.

Komponenten

Gelatine ist ein heterogenes Gemisch aus wasserlöslichen Proteinen mit hoher mittlerer Molekularmasse, das in Kollagen vorhanden ist. Die Proteine werden mittels Sieden der relevanten Haut, Sehnen, Ligamente, Knochen usw. in Wasser extrahiert. Gelatine vom Typ A wird aus mit Säure aufgeschlossenem Gewebe gewonnen. Gelatine vom Typ B wird aus mit Kalklauge geäschertem Gewebe gewonnen.

Vorsicht

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Angaben zur Herstellung

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)