NA2120

GenElute^™ Bacterial Genomic DNA Kits

sufficient for 350 purifications

• Synonyme(s) : Bacterial Genomic DNA, bacterial DNA prep, Gen Elute
• Code UNSPSC : 41105501
• Nomenclature NACRES : NA.55

Propriétés

• Utilisation: sufficient for 350 purifications
• Technique(s): DNA purification: suitable
• Température de stockage: 15-25°C

Description

Description générale

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified bacterial genomic DNA is ready for downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• cloning

The purified bacterial genomic DNA is ready for downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• cloning

GenElute^™ Bacterial Genomic DNA Kits has been used:

• to isolate DNA
• to prepare DNA from E. coli strain
• to extract genomic DNA

Caractéristiques et avantages

• Starting material: Up to 1.5 mL of culture
• Expected yield: Up to 20 μg
• Elution volume: 400 μl
• Time required: 70 - 120 min
• A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
• No phenol, chloroform, or ethanol precipitation required

• Typical DNA yields of 15 μg - 20 μg
• Protocols provided for Gram + and Gram - bacteria
• High quality genomic DNA in less than 2 hours
• Purified DNA has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9

Principe

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Autres remarques

For additional information, please see www.sigma-aldrich.com/genomicdna.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

• W0263 Wash Solution 1
• C2112 Column Preparation Solution
• P2308 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
• R6148 RNase A solution

Informations sur la sécurité

• Pictogrammes: GHS02,GHS08,GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H226,H290,H302,H315,H318,H334,H335,H336,H410
• Conseils de prudence: P210 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Central nervous system, Respiratory system
• Code de la classe de stockage: 3 - Flammable liquids
• Point d'éclair (°F): 77.0 °F - closed cup
• Point d'éclair (°C): 25 °C - closed cup