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NA2100

Sigma-Aldrich

GenElute™ Bacterial Genomic DNA Kits

sufficient for 10 purifications

Synonyme(s) :

Bacterial Genomic DNA, Gen Elute

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About This Item

Code UNSPSC :
41105501
Nomenclature NACRES :
NA.55

Utilisation

sufficient for 10 purifications

Niveau de qualité

200

Température de stockage

15-25°C

Catégories apparentées

Description générale

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

Caractéristiques et avantages

  • Starting material: Up to 1.5 mL of culture
  • Expected yield: Up to 20 μg
  • Elution volume: 400 μl
  • Time required: 70 - 120 min
  • A260/A280 ratio: 1.6 - 1.9
  • No phenol, chloroform, or ethanol precipitation required
  • Typical DNA yields of 15 μg - 20 μg
  • Protocols provided for Gram + and Gram - bacteria
  • High quality genomic DNA in less than 2 hours
  • Purified DNA has an A260/A280 ratio between 1.6 and 1.9

Principe

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Autres remarques

For additional information, please see www.sigma-aldrich.com/genomicdna.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • W0263Wash Solution 1FDS
  • C2112Column Preparation SolutionFDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologyFDS
  • R6148RNase A solutionFDS

En option

Réf. du produit
Description
Tarif

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

P2308

Protéinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

Agarose, BioReagent, for molecular biology, low EEO

T6400

Tampon Tris-borate-EDTA, 5× Concentrate

R6148

RNase A solution

NA2110

GenElute™ Bacterial Genomic DNA Kits, sufficient for 70 purifications

NA2120

GenElute™ Bacterial Genomic DNA Kits, sufficient for 350 purifications

C2112

Column Preparation Solution

W0263

Wash Solution 1

SRE0005

Protéinase K from Tritirachium album, Reagents designed and manufactured under current ISO 13485 certification.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Central nervous system, Respiratory system

Code de la classe de stockage

3 - Flammable liquids

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Certificats d'analyse (COA)

Lot/Batch Number
SLCH9555
SLCL8472
SLCK8228
SLCH9553
SLCH6677

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ivan M Mukisa et al.
International journal of food microbiology, 160(1), 1-10 (2012-11-13)
Obushera includes four fermented cereal beverages from Uganda namely: Obutoko, Enturire, Ekitiribita and Obuteire, whose microbial diversity has not hitherto been fully investigated. Knowledge of the microbial diversity and dynamics in these products is crucial for understanding their safety and
Pierre Stallforth et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(36), 14528-14533 (2013-07-31)
Stable multipartite mutualistic associations require that all partners benefit. We show that a single mutational step is sufficient to turn a symbiotic bacterium from an inedible but host-beneficial secondary metabolite producer into a host food source. The bacteria's host is
Lizbeth Sayavedra et al.
Environmental microbiology, 23(6), 3164-3181 (2021-04-21)
Sulfate-reducing bacteria (SRB) are widespread in human guts, yet their expansion has been linked to colonic diseases. We report the isolation, sequencing and physiological characterization of strain QI0027T , a novel SRB species belonging to the class Desulfovibrionia. Metagenomic sequencing
Cecilia A Silva et al.
Infection and immunity, 80(2), 839-849 (2011-11-16)
Salmonella enterica serovar Enteritidis causes a systemic, typhoid-like infection in newly hatched poultry and mice. In the present study, a library of 54,000 transposon mutants of S. Enteritidis phage type 4 (PT4) strain P125109 was screened for mutants deficient in
Susana Delgado et al.
Microbial ecology, 65(3), 763-772 (2013-02-12)
Stomach mucosa biopsies and gastric juices samples of 12 healthy persons were analysed by culturing in selective- and non-selective-rich media. Microbial DNA from four mucosal samples was also amplified by nested PCR using universal bacterial primers, and the 16S rDNA
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Protocoles

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

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