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NA2100

Sigma-Aldrich

GenElute™ Bacterial Genomic DNA Kits

sufficient for 10 purifications

Synonyme(s) :

Bacterial Genomic DNA, Gen Elute

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About This Item

Code UNSPSC :
41105501
Nomenclature NACRES :
NA.55

Utilisation

sufficient for 10 purifications

Température de stockage

15-25°C

Catégories apparentées

Description générale

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

Caractéristiques et avantages

  • Starting material: Up to 1.5 mL of culture
  • Expected yield: Up to 20 μg
  • Elution volume: 400 μl
  • Time required: 70 - 120 min
  • A260/A280 ratio: 1.6 - 1.9
  • No phenol, chloroform, or ethanol precipitation required
  • Typical DNA yields of 15 μg - 20 μg
  • Protocols provided for Gram + and Gram - bacteria
  • High quality genomic DNA in less than 2 hours
  • Purified DNA has an A260/A280 ratio between 1.6 and 1.9

Principe

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Autres remarques

For additional information, please see www.sigma-aldrich.com/genomicdna.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • W0263Wash Solution 1FDS
  • C2112Column Preparation SolutionFDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologyFDS
  • R6148RNase A solutionFDS

En option

Réf. du produit
Description
Tarif

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

P2308

Protéinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

Agarose, BioReagent, for molecular biology, low EEO

T6400

Tampon Tris-borate-EDTA, 5× Concentrate

R6148

RNase A solution

NA2110

GenElute™ Bacterial Genomic DNA Kits, sufficient for 70 purifications

NA2120

GenElute™ Bacterial Genomic DNA Kits, sufficient for 350 purifications

C2112

Column Preparation Solution

W0263

Wash Solution 1

SRE0005

Protéinase K from Tritirachium album, Reagents designed and manufactured under current ISO 13485 certification.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Central nervous system, Respiratory system

Code de la classe de stockage

3 - Flammable liquids

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Certificats d'analyse (COA)

Lot/Batch Number
SLCH9555
SLCL8472
SLCK8228
SLCH9553
SLCH6677

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Robert Murphy et al.
mSphere, 6(2) (2021-03-05)
Actinobacteria, one of the largest bacterial phyla, are ubiquitous in many of Earth's ecosystems and often act as defensive symbionts with animal hosts. Members of the phylum have repeatedly been isolated from basidiomycete-cultivating fungus-farming termites that maintain a monoculture fungus
K E Eboigbodin et al.
Applied microbiology and biotechnology, 73(3), 669-675 (2006-07-21)
The hypothesis tested in this paper is that quorum sensing influences the microbial surface electrokinetic properties. Escherichia coli MG1655 and MG1655 LuxS- mutant (lacking quorum-sensing gene for Autoinducer synthase AI-2) were used for this study. AI-2 production (or lack of)
Wafa Masoud et al.
International journal of food microbiology, 153(1-2), 192-202 (2011-12-14)
The purpose of this work was to study the bacterial communities in raw milk and in Danish raw milk cheeses using pyrosequencing of tagged amplicons of the V3 and V4 regions of the 16S rDNA and cDNA. Furthermore, the effects
Seyed Mohammad Mehdi Dastgheib et al.
Applied microbiology and biotechnology, 95(3), 789-798 (2011-11-17)
In this study we investigated the phenanthrene degradation by a halophilic consortium obtained from a saline soil sample. This consortium, named Qphe, could efficiently utilize phenanthrene in a wide range of NaCl concentrations, from 1% to 17% (w/v). Since none
Chityal Ganesh Kumar et al.
Biotechnology progress, 28(6), 1507-1516 (2012-09-11)
Eleven biosurfactant producing bacteria were isolated from different petroleum-contaminated soil and sludge samples. Among these 11 isolates, two were identified as promising, as they reduced the surface tension of culture medium to values below 27 mN m(-1) . Besides biosurfactant
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Protocoles

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

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